The invention discloses an ultrasonic emission adjusting device and method for a USICM, the weight is light, and the dynamic characteristic is good. The device comprises an ultrasonic transmitting module and a sample culture dish module, the ultrasonic transmitting module comprises a bottom plate, an ultrasonic transmitting head fixed on the bottom plate and an acoustic lens arranged on the ultrasonic transmitting head, and the sample culture dish module comprises a coil, a culture dish, a left side plate and a right side plate. The left side plate and the right side plate are both arc-shapedplates and are symmetrically fixed to the bottom plate, and threads are arranged on arc-shaped inner sides. The coil is in threaded fit with the threads of the left and right side plates. The culturedish is fixed on the coil, and a circular hole is formed in the center of the culture dish. According to the method, a prepared sample is sealed, bonded and fixed above the culture dish circular hole,then the culture dish with the coil is screwed on the left side plate and the right side plate, the vertical height of the culture dish is adjusted according to the inherent characteristics of the ultrasonic transmitting head and the acoustic lens and the specific requirements of an experiment, and the ultrasonic emission adjustment of the USICM is completed.