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CN1793927A 一种检测盐酸克伦特罗的试剂盒及其检测方法 无效 - 驳回 复审申请

一种检测盐酸克伦特罗的试剂盒及其检测方法
摘要：
一种检测盐酸克伦特罗的试剂盒及其检测方法，属于时间分辨荧光免疫分析(TRFIA)技术领域，用于对动物性食品、血液及尿液中盐酸克伦特罗(CBL)含量的检测。本发明配制的试剂盒，微孔板包被有CBL－OVA，加入CBL标准或样品，再加入CBL抗体。游离的CBL与微孔板上的CBL－OVA竞争CBL抗体，没有连接的CBL抗体被洗涤除去，加入EU3+－羊抗兔抗体，标记免疫反应后没有连接的EU3+－羊抗兔抗体被洗涤除去。加增强液后，用时间分辨荧光仪测定其荧光强度cps，荧光强度与样品中的CBL浓度成反比，对照标准曲线即可确定被测样品中CBL的含量。本发明提供的检测CBL试剂盒结构简单，使用方便、廉价、灵敏度高，最低检测浓度为小于0.05ng/ml，相应的最低检测量为小于25ng/kg。
摘要（英）：

A method for detecting CBL includes competing CBL antibody by tree CBL with CBL ¿C OVA on micro hole board, washing off unconnected CBL antibody, adding EU3+ - sheep resisting rabbit antibody and washing off unconnected EU3+ - sheep resisting rabbit antibody, adding intensified liquid and using time ¿C identification luminoscope to determine its fluorescence intensity, presenting fluorescence intensity to CBL concentration in sample to be inverse ratio, comparing with standard curve to obtain CBL content in sample. The reagent kit for realizing said method is also disclosed.

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