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发明公开
CN1978463A 间充质干细胞亲和肽的筛选与用途 失效 - 权利终止 许可

间充质干细胞亲和肽的筛选与用途
摘要：
本发明涉及细胞、多肽生物技术领域。本发明的目的在于筛选到一种与MSC强结合的小肽用于MSC的分离纯化及作为载体用于靶向治疗制剂的制备。本发明所述的MSC亲和肽序列为富含Ser、Thr、Asn、Lys的环七肽序列。本发明利用噬菌体展示技术通过与MSC进行亲和筛选，最终在随机环7肽库中筛选到能够和MSC强结合的小肽。利用本发明筛选到的小肽将其连接于固相支持物上可用于MSC的筛选、分离和纯化，与荧光标记物联接后可用于制备MSC的示踪剂，可作为MSC与药物间的载体可用于制备靶向治疗的制剂。本发明将在制备肿瘤靶向治疗药物、药物筛选、细胞体内示踪及细胞分选中具有极佳的应用前景，并将产生巨大的社会效益和经济效益。
摘要（英）：

This invention relates to biotechnology field of cell and polypeptide. The aim of this invention is screening a small peptide that can strongly combine with MSC, and using it to separate and purify MSC and using it as carrying agent to prepare targeted therapeutic agent. The stated binding peptide sequence of MSC is ringed seven peptide sequence that abundantly contains Ser, Thr, Asn, Lys. Finally this invention uses phage display technique to screen small peptide that can strongly combine with MSC in random libraries of ringed seven peptides by affinity screening with MSC. MSC can link to solid phase sustentaculum through small peptide screened by using this invention. It can be used for screening, separating and purifying MSC. It can be used to prepare tracer agent of MSC after linking with fluorescent tumor marker. It can be used as carrying agent of MSC and medicine to prepare praeparatum about targeted therapy.

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