The utility model provides an image acquisition system belongs to image acquisition technical field. This image acquisition system includes controlling means, projecting apparatus and camera. Controlling means includes display card and image acquisition card, the display card with the projecting apparatus coupling, image acquisition card with the camera coupling, the display card is including the vertical synchronizing signal output that is used for exporting vertical synchronizing signal, the vertical synchronizing signal output with the outside lock input end of camera is through the interface circuit coupling. The utility model provides an image acquisition system has improved the image that the scintillation of fluorescent lamp was launched the projecting apparatus in the environment effectively and has led to the fact the relatively poor problem of quality that results in the camera to take a candid photograph the image of disturbing, has just improved image acquisition's speed.