发明公开
EP0379428A1 Improved method for the expression of biotin in bacillus sphaericus
失效
Verfahren zur表达von生物素在球形芽孢杆菌。
- 专利标题： Improved method for the expression of biotin in bacillus sphaericus
- 专利标题（中）： Verfahren zur表达von生物素在球形芽孢杆菌。
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申请号： EP90400129.4申请日： 1990-01-17
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公开(公告)号： EP0379428A1公开(公告)日： 1990-07-25
- 发明人: Speck, Denis , Gloeckler, Rémi , Lemoine, Yves , Ohsawa, Ikuro , Kisou, Tugio , Kamogawa, Kouichi
- 申请人： TRANSGENE S.A. , NIPPON ZEON CO., LTD.
- 申请人地址： 11, rue de Molsheim 67000 Strasbourg FR
- 专利权人： TRANSGENE S.A.,NIPPON ZEON CO., LTD.
- 当前专利权人： TRANSGENE S.A.,NIPPON ZEON CO., LTD.
- 当前专利权人地址： 11, rue de Molsheim 67000 Strasbourg FR
- 代理机构： Warcoin, Jacques
- 优先权： FR8900597 19890119
- 主分类号： C12N15/01
- IPC分类号： C12N15/01 ; C12N15/52 ; C12P17/18
摘要：
The present invention relates to a method for the selection of organisms derepressed in the expression of primary or secondary metabolites, starting with a parent strain of this normally repressed organism, wherein:
- said parent strain is transformed with a plasmid carrying a marker gene under the control of the DNA sequence involved in the regulation of the expression of said metabolite,
- the strains thus transformed are subjected to a mutagenic treatment, and
- the positive strains expressing said marker in the presence of said metabolite at a concentration totally repressing the expression of said marker in the transformed parent strain are selected,
- from among these positive strains, the strains hyperproductive of metabolite are selected, optionally after they have been relieved of their plasmids.
- said parent strain is transformed with a plasmid carrying a marker gene under the control of the DNA sequence involved in the regulation of the expression of said metabolite,
- the strains thus transformed are subjected to a mutagenic treatment, and
- the positive strains expressing said marker in the presence of said metabolite at a concentration totally repressing the expression of said marker in the transformed parent strain are selected,
- from among these positive strains, the strains hyperproductive of metabolite are selected, optionally after they have been relieved of their plasmids.
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