发明公开
EP1365643A2 MICROINJECTION ASSEMBLY AND METHODS FOR MICROINJECTING AND REIMPLANTING AVIAN EGGS
有权
“微注射装置和方法微注塑和韵律人工林鸟蛋”
- 专利标题: MICROINJECTION ASSEMBLY AND METHODS FOR MICROINJECTING AND REIMPLANTING AVIAN EGGS
- 专利标题(中): “微注射装置和方法微注塑和韵律人工林鸟蛋”
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申请号: EP02718905.9申请日: 2002-02-06
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公开(公告)号: EP1365643A2公开(公告)日: 2003-12-03
- 发明人: CHRISTMANN, Leandro
- 申请人: Avigenics, Inc.
- 申请人地址: 111 Riverbend Road Athens, GA 30605 US
- 专利权人: Avigenics, Inc.
- 当前专利权人: Avigenics, Inc.
- 当前专利权人地址: 111 Riverbend Road Athens, GA 30605 US
- 代理机构: Silveston, Judith
- 优先权: US269012P 20010213; US919143 20010731
- 国际公布: WO02064727 20020822
- 主分类号: A01K45/00
- IPC分类号: A01K45/00 ; A61K48/00 ; C12N15/00
摘要:
The present invention provides a microinjection assembly including a microscope, a microinjection system comprising a micromanipulator, a micropipette and a piezo-electric oscillator, and an obliquely angled macro monitoring unit. The present invention also provides methods of microinjecting the germinal disk of an avian egg, thereby delivering a transgenic nucleus, spermatozoon or isolated nucleic acid to the avian embryo. The avian ovum may be returned to a female bird for hard-shell deposit and laying of the egg for hatching as a transfected bird.
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