发明公开
- 专利标题： HIGH THROUGHPUT MUTAGENESIS SCREENING METHOD
- 专利标题（中）： 具有高吞吐量诱变筛选过程
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申请号： EP03744218申请日： 2003-03-05
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公开(公告)号： EP1543117A4公开(公告)日： 2007-06-20
- 发明人: BOTT RICHARD R , KELLIS JAMES T JR , MORRISON THOMAS B
- 申请人： GENENCOR INT
- 专利权人： GENENCOR INT
- 当前专利权人： GENENCOR INT
- 优先权： US9191202 2002-03-05; US9222702 2002-03-05; US36237202 2002-03-05; US40392102 2002-08-15
- 主分类号： C12N9/18
- IPC分类号： C12N9/18 ; C12N9/20
摘要：
The present invention provides mutagenesis methods for protein engineering. In particular, the present inventionprovides mutagenesis methods that include feedback adjustment from systematic result evaluation. More specifically, the invention provides site-saturation mutagenesis methods that screen for variants with one or more desirable protein properties and evaluates screening results to provide feedback for repeat screening and construction of new libraries. The invention further provides cutinase variants that provide improved stability and activity compared with the wild type cutinase.
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