公开(公告)号：EP1543117A4

公开(公告)日：2007-06-20

申请号：EP03744218

申请日：2003-03-05

申请人： GENENCOR INT

发明人： BOTT RICHARD R ,  KELLIS JAMES T JR ,  MORRISON THOMAS B

IPC分类号： C12N9/18 ,  C12N9/20

CPC分类号： C12Y301/01074 ,  C12N9/18

摘要： The present invention provides mutagenesis methods for protein engineering. In particular, the present inventionprovides mutagenesis methods that include feedback adjustment from systematic result evaluation. More specifically, the invention provides site-saturation mutagenesis methods that screen for variants with one or more desirable protein properties and evaluates screening results to provide feedback for repeat screening and construction of new libraries. The invention further provides cutinase variants that provide improved stability and activity compared with the wild type cutinase.