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1.发明公开MUTANT E. COLI APPA PHYTASE ENZYMES AND NATURAL VARIANTS THEREOF, NUCLEIC ACIDS ENCODING SUCH PHYTASE ENZYMES, VECTORS AND HOST CELLS INCORPORATING SAME AND METHODS OF MAKING AND USING SAME 有权大肠杆菌突变株APPA植酸酶的酶和天然变异物，例如肌醇六磷酸酶的核酸编码，将含有载体和宿主细胞AND METHOD FOR PRODUCTION及其用途
公开(公告)号：EP1546316A4
公开(公告)日：2007-05-02
申请号：EP03785159
申请日：2003-08-11
申请人： GENENCOR INT
发明人： BLATTMANN BEAT O , DARZINS ALDIS , DAVIS JOHN M , ENCELL LANCE P , MORRISON THOMAS B , MRACHKO GREGORY T , SCHELLENBERGER VOLKER
CPC分类号： C12N15/8243 , C12N9/16 , C12N15/8245
摘要： Cells producing mutant phytases having modified activity are provided, as well as the phytases so produced. Also provided are methods of making and producing such phytases and the use of the expressed phytase protein in feed as a supplement.
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公开(公告)号：EP1325457A4
公开(公告)日：2007-10-24
申请号：EP01979689
申请日：2001-10-10
申请人： GENENCOR INT
IPC分类号： C12N9/04 , C12N9/16 , C12N9/24 , C12N9/50 , C12P19/34 , C12Q1/68 , C40B30/02 , G06F19/16 , G06F19/22 , G06F19/00
摘要： Methods of creating libraries of biological polymers are provided. The construction of a library employs a probability matrix for a reference sequence, and a constraint vector for which is applied to the probability matirx to produce a substitution scheme. Thesubstitution scheme is then used to generate a library comprisingsubstitutions recommended by the substitution scheme. The librarymembers, or host cells comprising and/or expressing them, can be screened for desired changes in a property of interest in the biological polymers in the library.
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公开(公告)号：EP1543117A4
公开(公告)日：2007-06-20
申请号：EP03744218
申请日：2003-03-05
申请人： GENENCOR INT
CPC分类号： C12Y301/01074 , C12N9/18
摘要： The present invention provides mutagenesis methods for protein engineering. In particular, the present inventionprovides mutagenesis methods that include feedback adjustment from systematic result evaluation. More specifically, the invention provides site-saturation mutagenesis methods that screen for variants with one or more desirable protein properties and evaluates screening results to provide feedback for repeat screening and construction of new libraries. The invention further provides cutinase variants that provide improved stability and activity compared with the wild type cutinase.
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4.发明公开
公开(公告)号：EP1412513A4
公开(公告)日：2005-04-27
申请号：EP02803271
申请日：2002-07-26
申请人： GENENCOR INT
发明人： COLLIER KATHERINE D , LEE EDWIN , LEIVA NICHOLAS , MORRISON THOMAS B , SCHELLENBERGER VOLKER , SELIFONOVA OLGA
IPC分类号： C07K14/32 , C12N15/75 , C12P21/02 , C12P21/04 , C12P21/06 , C07H21/02 , C07H21/04 , C12N1/20 , C12N15/00 , C12N15/09 , C12N15/63 , C12N15/70 , C12N15/74 , C12R1/125
摘要： An evolvable production strain of B. subtilis exhibiting continuous or high level expression during protein evolution is described. An evolved Bacillus subtilis pstS promoter facilitates screening and production of secreted proteins.
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