发明公开
EP1717588A1 Measuring thrombin activity in whole blood
审中-公开
Volblut的Bestimmung derThrombinaktivität
- 专利标题： Measuring thrombin activity in whole blood
- 专利标题（中）： Volblut的Bestimmung derThrombinaktivität
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申请号： EP05290952.0申请日： 2005-04-29
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公开(公告)号： EP1717588A1公开(公告)日： 2006-11-02
- 发明人: Hemker, Hendrik Coenraad , Al-Dieri, Raed , Nijhuis, Sebastiaan , Beguin, Suzette , Wagenvoord, Robert , Giesen, Peter
- 申请人： Synapse B.V.
- 申请人地址： Universiteitsingel 50 6229 ER Maastricht NL
- 专利权人： Synapse B.V.
- 当前专利权人： Synapse B.V.
- 当前专利权人地址： Universiteitsingel 50 6229 ER Maastricht NL
- 代理机构： Desaix, Anne
- 主分类号： G01N33/86
- IPC分类号： G01N33/86
摘要：
The invention relates to a method for in vitro determining thrombin activity in a sample wherein the sample is a blood sample and thrombin generation is measured by the steps of:
- contacting a layer of said sample with a fluorogenic substrate of thrombin, wherein said layer has a thickness within a range of 0.05 to 5 mm and a surface within a range of 10 to 500 mm 2 ;
- allowing thrombin to generate in said sample;
- measuring the fluorescence emitted from the surface of the layer, by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on said fluorogenic substrate.
- contacting a layer of said sample with a fluorogenic substrate of thrombin, wherein said layer has a thickness within a range of 0.05 to 5 mm and a surface within a range of 10 to 500 mm 2 ;
- allowing thrombin to generate in said sample;
- measuring the fluorescence emitted from the surface of the layer, by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on said fluorogenic substrate.
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