公开(公告)号：EP1717588A1

公开(公告)日：2006-11-02

申请号：EP05290952.0

申请日：2005-04-29

申请人： Synapse B.V.

发明人： Hemker, Hendrik Coenraad ,  Al-Dieri, Raed ,  Nijhuis, Sebastiaan ,  Beguin, Suzette ,  Wagenvoord, Robert ,  Giesen, Peter

IPC分类号： G01N33/86

CPC分类号： C12Q1/56 ,  G01N2333/974

摘要： The invention relates to a method for in vitro determining thrombin activity in a sample wherein the sample is a blood sample and thrombin generation is measured by the steps of:
- contacting a layer of said sample with a fluorogenic substrate of thrombin, wherein said layer has a thickness within a range of 0.05 to 5 mm and a surface within a range of 10 to 500 mm 2 ;
- allowing thrombin to generate in said sample;
- measuring the fluorescence emitted from the surface of the layer, by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on said fluorogenic substrate.

摘要翻译： 本发明涉及一种用于体外测定样品中凝血酶活性的方法，其中所述样品是血液样品，并且通过以下步骤测量凝血酶产生： - 使所述样品层与凝血酶的荧光底物接触，其中所述层具有 厚度在0.05〜5mm的范围内，表面在10〜500mm 2的范围内; - 允许凝血酶在所述样品中产生; 由荧光基质释放的荧光基团，由于所产生的凝血酶在所述荧光底物上的酶促作用而测量从该表面发射的荧光。