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    Measuring thrombin activity in whole blood
    3.
    发明公开
    Measuring thrombin activity in whole blood 审中-公开
    Volblut的Bestimmung derThrombinaktivität

    公开(公告)号：EP1717588A1

    公开(公告)日：2006-11-02

    申请号：EP05290952.0

    申请日：2005-04-29

    申请人： Synapse B.V.

    发明人： Hemker, Hendrik Coenraad ,  Al-Dieri, Raed ,  Nijhuis, Sebastiaan ,  Beguin, Suzette ,  Wagenvoord, Robert ,  Giesen, Peter

    IPC分类号： G01N33/86

    CPC分类号： C12Q1/56 ,  G01N2333/974

    摘要： The invention relates to a method for in vitro determining thrombin activity in a sample wherein the sample is a blood sample and thrombin generation is measured by the steps of:
    - contacting a layer of said sample with a fluorogenic substrate of thrombin, wherein said layer has a thickness within a range of 0.05 to 5 mm and a surface within a range of 10 to 500 mm 2 ;
    - allowing thrombin to generate in said sample;
    - measuring the fluorescence emitted from the surface of the layer, by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on said fluorogenic substrate.

    摘要翻译： 本发明涉及一种用于体外测定样品中凝血酶活性的方法，其中所述样品是血液样品，并且通过以下步骤测量凝血酶产生： - 使所述样品层与凝血酶的荧光底物接触，其中所述层具有 厚度在0.05〜5mm的范围内，表面在10〜500mm 2的范围内; - 允许凝血酶在所述样品中产生; 由荧光基质释放的荧光基团，由于所产生的凝血酶在所述荧光底物上的酶促作用而测量从该表面发射的荧光。

    Time-course measurement of enzymatic activity corrected for impacts of disturbances relating to the reaction of the enzyme with a substrate
    5.
    发明公开
    Time-course measurement of enzymatic activity corrected for impacts of disturbances relating to the reaction of the enzyme with a substrate 有权
    为了研究在该反应的底物的酶的酶活性的时间过程中的任何干扰校正的测量的影响

    公开(公告)号：EP2088435A1

    公开(公告)日：2009-08-12

    申请号：EP08151181.8

    申请日：2008-02-07

    申请人： Synapse B.V.

    发明人： Hemker, Hendrik Coenraad ,  Hemker, Pieter Wilhelm

    IPC分类号： G01N33/86 ,  C12Q1/37 ,  C12Q1/56

    CPC分类号： C12Q1/56 ,  C12Q1/37 ,  G01N2333/974

    摘要： The invention relates to time-course measurement of enzymatic activity corrected for impacts of disturbances relating to the reaction of the enzyme with a substrate.
    The in vitro method of the invention is especially intended for the measurement of clotting or the fibrinolysis activity in vitro .
    The invention enables to measure the exact time course of an enzymatic activity that develops and/or disappears in a reaction mixture, especially in a blood sample.
    The invention thus relates to a method of determination of the course of an enzyme activity that is variable in time, wherein said activity is probed by conversion of a substrate of the enzyme, comprising, in a selected test set up and for a determined substrate of the enzyme, the determination of the velocity of signal production (dF diag /dt) resulting from a time curve of the signal (F diao =f(A)) obtained from splitting said substrate when it is contacted with a determined initially fixed concentration of the enzyme (E) and providing a "diagnostic plot" with the values of (dF diag /dt) against the signal (F diag ) and determining whether said diagnostic plot is either a straight line or a parabola and in the same test conditions, for a given test sample, the determination of the signal production (F exp ) resulting from splitting the substrate by the enzyme generating in and/or disappearing from the sample and providing the time curve of signal F exp =f(t); and transforming the obtained experimental value of the signal (F exp ) into an ideal value (F transf )

    摘要翻译： 本发明涉及一种用于干扰涉及所述酶的与底物反应的影响校正酶活性的时间过程的测量。 本发明的体外方法的目的是尤其是对凝血的测量或体外纤维蛋白溶解活性的爱。 本发明能够测量的酶活性的确切时间进程没有开发出和/或消失在反应混合物中，爱尤其是血液样品。 因此，本发明涉及确定的酶活性的过程的方法所做的是在时间上可变的，worin所述活性是通过设置了酶，其包括，在所选择的测试的底物的转化率和对的确定性开采衬底探测 酶，信号生成（DF DIAG / dt）的速度的确定从分束而获得的信号（F刁= F（A））的一个时间曲线得到的所述基板当它与的确定性开采INITIALLY固定浓度接触 酶（e）和提供“诊断图”与针对信号（F DIAG）的值（DF DIAG / DT）和确定性采矿是否所述诊断图可以是一个直线或抛物线，并在相同的试验条件， 对于给定的测试样品中，信号生成（F EXP）从由酶产生中和/或从样品中消失，并提供信号F EXP = F（t）的时间曲线分割衬底得到的测定; 和将所述信号（F EXP）的所获得的实验值成理想值（F TRANSF）