发明公开
EP2218780A1 NUCLEIC ACID AMPLIFICATION METHOD, AND REAGENT AND REAGENT KIT FOR USE IN THE METHOD
有权
核酸扩增方法，以及用于该方法的试剂和试剂盒
- 专利标题： NUCLEIC ACID AMPLIFICATION METHOD, AND REAGENT AND REAGENT KIT FOR USE IN THE METHOD
- 专利标题（中）： 核酸扩增方法，以及用于该方法的试剂和试剂盒
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申请号： EP08839845.8申请日： 2008-10-17
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公开(公告)号： EP2218780A1公开(公告)日： 2010-08-18
- 发明人: YONEKAWA, Toshihiro , NOTOMI, Tsugunori , KANDA, Hidetoshi , HOSAKA, Norimitsu
- 申请人： Eiken Kagaku Kabushiki Kaisha
- 申请人地址： 19-9 Taito 4-chome Taito-ku Tokyo 110-8408 JP
- 专利权人： Eiken Kagaku Kabushiki Kaisha
- 当前专利权人： Eiken Kagaku Kabushiki Kaisha
- 当前专利权人地址： 19-9 Taito 4-chome Taito-ku Tokyo 110-8408 JP
- 代理机构： Schwahn, Hartmut
- 优先权： JP2007271902 20071019
- 国际公布： WO2009051214 20090423
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12Q1/68
摘要：
The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.
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