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    NUCLEIC ACID AMPLIFICATION METHOD, AND REAGENT AND REAGENT KIT FOR USE IN THE METHOD
    3.
    发明公开
    NUCLEIC ACID AMPLIFICATION METHOD, AND REAGENT AND REAGENT KIT FOR USE IN THE METHOD 有权
    核酸扩增方法，以及用于该方法的试剂和试剂盒

    公开(公告)号：EP2218780A1

    公开(公告)日：2010-08-18

    申请号：EP08839845.8

    申请日：2008-10-17

    申请人： Eiken Kagaku Kabushiki Kaisha

    发明人： YONEKAWA, Toshihiro ,  NOTOMI, Tsugunori ,  KANDA, Hidetoshi ,  HOSAKA, Norimitsu

    IPC分类号： C12N15/09 ,  C12Q1/68

    CPC分类号： C12Q1/6876 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2527/143 ,  C12Q2527/113 ,  C12Q2545/101 ,  C12Q2537/149 ,  C12Q2531/119

    摘要： The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.

    摘要翻译： 本发明的目的是提供一种方法，该方法允许内标物质的稳定扩增，同时在涉及使用内标物质和使用的试剂盒的核酸检测系统中维持靶核酸的准确测定值 为此。 本发明涉及核酸扩增方法，其包括通过在扩增靶的方法中在扩增靶核酸之前进行内标物质的扩增来防止内标扩增产物影响靶核酸的扩增反应 使用内部标准物质的样品中的核酸以及为此使用的试剂和试剂盒。

    COMPOUND CONTAINER AND POURING-OUT METHOD
    5.
    发明公开
    COMPOUND CONTAINER AND POURING-OUT METHOD 有权
    VERBUNDBEHÄLTERUND AUSGIESSVERFAHRENDAFÜR

    公开(公告)号：EP2279965A1

    公开(公告)日：2011-02-02

    申请号：EP09735976.4

    申请日：2009-03-03

    申请人： Toyo Seikan Kaisha, Ltd. ,  EIKEN KAGAKU KABUSHIKI KAISHA

    发明人： SHIBATA, Tomoyuki ,  MASUYA, Natsuki ,  SETO, Yoshiaki ,  SAITO, Shingo ,  MORI, Yasuyoshi ,  KUBOTA, Yutaka ,  KANDA, Hidetoshi ,  NOTOMI, Tsugunori ,  KANADA, Teijirou ,  TAKANO, Youichi ,  TAKEUCHI, Yasuyuki ,  ONISHI, Akira

    IPC分类号： B65D81/32 ,  B65D51/28

    CPC分类号： B65D47/06 ,  B65D51/222 ,  B65D51/226 ,  B65D51/227 ,  B65D51/285 ,  B65D2251/0025 ,  B65D2251/0093 ,  B65D2251/0096 ,  Y10T436/2575

    摘要： A compound container which is comprised of a container main body 2 forming a first accommodation chamber 20 and an auxiliary container 3 forming a second accommodation chamber 30, wherein a mounting part 22 on which the auxiliary container 3 is mounted is provided in the container main body 2 and a cutting part 221 which cuts a second accommodation chamber partition wall 30a which partitions part of the second accommodation chamber 30 formed in the auxiliary container 3 is formed, and when the auxiliary container 3 is mounted on the container main body 2, the second accommodation chamber partition wall 30a is cut, whereby the first accommodation chamber 20 and the second accommodation chamber 30 are intercommunicated. As a result, if contents which are needed to be heat-treated are heated while keeping the state where the contents are sealed within the container, the contents which are sealed in a plurality of accommodation chambers formed in the container can be mixed while keeping the contents being isolated from the outside environment by effectively preventing the contents to be heated from leaking outside as well as by preventing deterioration of sealing performance.

    摘要翻译： 复合容器，其由形成第一容纳室20的容器主体2和形成第二容纳室30的辅助容器3构成，其中安装有辅助容器3的安装部22设置在容器主体 2和切割部分221，其切割分隔形成在辅助容器3中的第二容纳室30的一部分的第二容纳室分隔壁30a，并且当辅助容器3安装在容器主体2上时，第二 收纳室分隔壁30a被切断，由此第一容纳室20和第二容纳室30相互连通。 结果，如果需要进行热处理的内容物在保持内容物被密封在容器内的状态的同时被加热，则密封在形成在容器中的多个容纳室中的内容物可以混合，同时保持 内容物通过有效地防止内容物被加热泄漏到外部以及防止密封性能的劣化而与外部环境隔离。

    METHOD AND KIT FOR PREPARATION OF SAMPLE FOR USE IN NUCLEIC ACID AMPLIFICATION
    7.
    发明公开
    METHOD AND KIT FOR PREPARATION OF SAMPLE FOR USE IN NUCLEIC ACID AMPLIFICATION 有权
    VERFAHREN UND KITFÜRDIE ZUBEREITUNG EINER PROBE ZURNUKLEINSÄUREAMPLIFIKATION

    公开(公告)号：EP2218791A1

    公开(公告)日：2010-08-18

    申请号：EP08847811.0

    申请日：2008-11-05

    申请人： EIKEN KAGAKU KABUSHIKI KAISHA

    发明人： MORI, Yasuyoshi ,  NOTOMI, Tsugunori ,  SHINDOME, Tsuyoshi

    IPC分类号： C12Q1/68 ,  C12N15/09

    CPC分类号： C12N15/1006

    摘要： The object is to remove substances that can inhibit nucleic acid amplification from biological samples and nucleic acid extracts, and to allow convenient and accurate evaluation of the presence or absence of a target nucleic acid or the expression level of a target gene in a biological sample, by nucleic acid amplification means employing an enzyme. The invention provides a method for preparation of a sample for use in nucleic acid amplification, to be used for amplification of nucleic acid in a biological sample, which method comprises an extraction step in which a nucleic acid extraction reagent is added to the biological sample to obtain a nucleic acid extract, and a purification step in which the nucleic acid extract is contacted with zeolite to obtain a nucleic acid amplification sample suitable for nucleic acid amplification, to allow nucleic acid detection at high sensitivity.

    摘要翻译： 本发明的目的是去除能够抑制生物样品和核酸提取物的核酸扩增的物质，并且允许方便和准确地评估靶核酸的存在或不存在或生物样品中靶基因的表达水平， 通过使用酶的核酸扩增方法。 本发明提供了一种制备用于核酸扩增的样品的方法，用于生物样品中的核酸扩增，该方法包括提取步骤，其中将核酸提取试剂加入生物样品至 获得核酸提取物和纯化步骤，其中核酸提取物与沸石接触以获得适合于核酸扩增的核酸扩增样品，以允许高灵敏度的核酸检测。