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    FULLY AUTOMATIC GENETIC TESTING DEVICE
    1.
    发明公开
    FULLY AUTOMATIC GENETIC TESTING DEVICE 审中-公开

    公开(公告)号：EP3778851A1

    公开(公告)日：2021-02-17

    申请号：EP19774406.3

    申请日：2019-03-29

    申请人： EIKEN KAGAKU KABUSHIKI KAISHA

    发明人： SEGAWA, Yuji ,  WATANABE, Hidetoshi ,  YONEKAWA, Toshihiro ,  KAZUMI, Fumihiko ,  PETERSON, Rod ,  MOSKALEV, Anatoly ,  FORSHAGER, Goran ,  FOSTER, James

    IPC分类号： C12M1/00 ,  C12Q1/6844

    摘要： The present invention aims to provide a compact, fully automatic genetic testing device with which a step of extracting and purifying a nucleic acid from a sample, a step of injecting a solution containing the nucleic acid, and a step of amplifying and detecting the nucleic acid, can be fully automatically carried out, and which enables a physician to carry out genetic testing at a position close to a patient. The fully automatic genetic testing device comprises: a first unit for extracting and purifying a nucleic acid from a sample; a second unit for injecting a solution containing the nucleic acid to a testing chip having reaction sites for amplification of the nucleic acid; and a third unit for optically detecting the nucleic acid amplified in the reaction sites.

    NUCLEIC ACID EXTRACTION METHOD AND KIT USING SAME
    2.
    发明公开
    NUCLEIC ACID EXTRACTION METHOD AND KIT USING SAME 审中-公开

    公开(公告)号：EP3521447A1

    公开(公告)日：2019-08-07

    申请号：EP17855832.6

    申请日：2017-09-19

    申请人： Eiken Kagaku Kabushiki Kaisha

    发明人： SATO, Masaki ,  YONEKAWA, Toshihiro

    IPC分类号： C12Q1/68 ,  C12N15/09

    摘要： A method for extracting nucleic acid from a biological sample is disclosed. The method comprises steps of: (i) mixing a biological sample, an anionic surfactant and a chaotropic compound to obtain a lysate; (ii) mixing the lysate, an alkanol having a boiling point higher than 75°C, a chaotropic compound and silica particles to adsorb the nucleic acid on the silica particles; (iii) washing the silica particles having the nucleic acid adsorbed thereon with a washing liquid; and (iv) eluting the nucleic acid adsorbed on the silica particles with an eluent, wherein at least steps (i) and (ii) are performed at a temperature higher than 75°C.

    NUCLEIC ACID AMPLIFICATION METHOD, AND REAGENT AND REAGENT KIT FOR USE IN THE METHOD
    4.
    发明公开
    NUCLEIC ACID AMPLIFICATION METHOD, AND REAGENT AND REAGENT KIT FOR USE IN THE METHOD 有权
    核酸扩增方法，以及用于该方法的试剂和试剂盒

    公开(公告)号：EP2218780A1

    公开(公告)日：2010-08-18

    申请号：EP08839845.8

    申请日：2008-10-17

    申请人： Eiken Kagaku Kabushiki Kaisha

    发明人： YONEKAWA, Toshihiro ,  NOTOMI, Tsugunori ,  KANDA, Hidetoshi ,  HOSAKA, Norimitsu

    IPC分类号： C12N15/09 ,  C12Q1/68

    CPC分类号： C12Q1/6876 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2527/143 ,  C12Q2527/113 ,  C12Q2545/101 ,  C12Q2537/149 ,  C12Q2531/119

    摘要： The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.

    摘要翻译： 本发明的目的是提供一种方法，该方法允许内标物质的稳定扩增，同时在涉及使用内标物质和使用的试剂盒的核酸检测系统中维持靶核酸的准确测定值 为此。 本发明涉及核酸扩增方法，其包括通过在扩增靶的方法中在扩增靶核酸之前进行内标物质的扩增来防止内标扩增产物影响靶核酸的扩增反应 使用内部标准物质的样品中的核酸以及为此使用的试剂和试剂盒。