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    METHOD FOR DETECTING TARGET BASE SEQUENCE, METHOD FOR DESIGNING AND PRODUCING PROBE, AND KIT
    1.
    发明公开
    METHOD FOR DETECTING TARGET BASE SEQUENCE, METHOD FOR DESIGNING AND PRODUCING PROBE, AND KIT 审中-公开

    公开(公告)号：EP3533880A1

    公开(公告)日：2019-09-04

    申请号：EP17865651.8

    申请日：2017-10-25

    申请人： Eiken Kagaku Kabushiki Kaisha

    发明人： MICHIYUKI, Satoru ,  KANDA, Hidetoshi

    IPC分类号： C12Q1/68 ,  G01N21/78 ,  C12N15/09

    摘要： The present invention addresses the issue of providing a target base sequence detection method, etc., whereby a determination can be readily made regarding whether or not a target base sequence is present in a nucleic acid sample. A fluorescent-labeled detection probe and a competitive probe are added to a nucleic acid sample and caused to hybridize with the nucleic acid in the sample, the fluorescence intensity is measured while changing the temperature of the reaction sample, and first order differentiation is performed on a temperature- fluorescence intensity curve. The fluorescent-labeled detection probe and competitive probe base length, base sequence, and amount to be added to nucleic acid samples are determined such that the first order derivative curve for a control target reaction sample including a target base sequence has a peak but the first order derivative curve for a control non-target reaction sample including a non-target base sequence does not substantially have a peak, when: the fluorescent-labeled detection probe and the competitive probe are added to both the control target nucleic acid sample and the control non-target nucleic acid sample; the fluorescence intensity is measured while the temperature of both the obtained control target reaction sample and the control non-target reaction sample are changed; and first order differentiation is performed on a temperature- fluorescence intensity curve.

    COMPOUND CONTAINER AND POURING-OUT METHOD
    2.
    发明公开
    COMPOUND CONTAINER AND POURING-OUT METHOD 有权
    VERBUNDBEHÄLTERUND AUSGIESSVERFAHRENDAFÜR

    公开(公告)号：EP2279965A1

    公开(公告)日：2011-02-02

    申请号：EP09735976.4

    申请日：2009-03-03

    申请人： Toyo Seikan Kaisha, Ltd. ,  EIKEN KAGAKU KABUSHIKI KAISHA

    发明人： SHIBATA, Tomoyuki ,  MASUYA, Natsuki ,  SETO, Yoshiaki ,  SAITO, Shingo ,  MORI, Yasuyoshi ,  KUBOTA, Yutaka ,  KANDA, Hidetoshi ,  NOTOMI, Tsugunori ,  KANADA, Teijirou ,  TAKANO, Youichi ,  TAKEUCHI, Yasuyuki ,  ONISHI, Akira

    IPC分类号： B65D81/32 ,  B65D51/28

    CPC分类号： B65D47/06 ,  B65D51/222 ,  B65D51/226 ,  B65D51/227 ,  B65D51/285 ,  B65D2251/0025 ,  B65D2251/0093 ,  B65D2251/0096 ,  Y10T436/2575

    摘要： A compound container which is comprised of a container main body 2 forming a first accommodation chamber 20 and an auxiliary container 3 forming a second accommodation chamber 30, wherein a mounting part 22 on which the auxiliary container 3 is mounted is provided in the container main body 2 and a cutting part 221 which cuts a second accommodation chamber partition wall 30a which partitions part of the second accommodation chamber 30 formed in the auxiliary container 3 is formed, and when the auxiliary container 3 is mounted on the container main body 2, the second accommodation chamber partition wall 30a is cut, whereby the first accommodation chamber 20 and the second accommodation chamber 30 are intercommunicated. As a result, if contents which are needed to be heat-treated are heated while keeping the state where the contents are sealed within the container, the contents which are sealed in a plurality of accommodation chambers formed in the container can be mixed while keeping the contents being isolated from the outside environment by effectively preventing the contents to be heated from leaking outside as well as by preventing deterioration of sealing performance.

    摘要翻译： 复合容器，其由形成第一容纳室20的容器主体2和形成第二容纳室30的辅助容器3构成，其中安装有辅助容器3的安装部22设置在容器主体 2和切割部分221，其切割分隔形成在辅助容器3中的第二容纳室30的一部分的第二容纳室分隔壁30a，并且当辅助容器3安装在容器主体2上时，第二 收纳室分隔壁30a被切断，由此第一容纳室20和第二容纳室30相互连通。 结果，如果需要进行热处理的内容物在保持内容物被密封在容器内的状态的同时被加热，则密封在形成在容器中的多个容纳室中的内容物可以混合，同时保持 内容物通过有效地防止内容物被加热泄漏到外部以及防止密封性能的劣化而与外部环境隔离。

    NUCLEIC ACID AMPLIFICATION METHOD, AND REAGENT AND REAGENT KIT FOR USE IN THE METHOD
    5.
    发明公开
    NUCLEIC ACID AMPLIFICATION METHOD, AND REAGENT AND REAGENT KIT FOR USE IN THE METHOD 有权
    核酸扩增方法，以及用于该方法的试剂和试剂盒

    公开(公告)号：EP2218780A1

    公开(公告)日：2010-08-18

    申请号：EP08839845.8

    申请日：2008-10-17

    申请人： Eiken Kagaku Kabushiki Kaisha

    发明人： YONEKAWA, Toshihiro ,  NOTOMI, Tsugunori ,  KANDA, Hidetoshi ,  HOSAKA, Norimitsu

    IPC分类号： C12N15/09 ,  C12Q1/68

    CPC分类号： C12Q1/6876 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2527/143 ,  C12Q2527/113 ,  C12Q2545/101 ,  C12Q2537/149 ,  C12Q2531/119

    摘要： The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.

    摘要翻译： 本发明的目的是提供一种方法，该方法允许内标物质的稳定扩增，同时在涉及使用内标物质和使用的试剂盒的核酸检测系统中维持靶核酸的准确测定值 为此。 本发明涉及核酸扩增方法，其包括通过在扩增靶的方法中在扩增靶核酸之前进行内标物质的扩增来防止内标扩增产物影响靶核酸的扩增反应 使用内部标准物质的样品中的核酸以及为此使用的试剂和试剂盒。

    CONTAINER FOR INSPECTION
    7.
    发明公开
    CONTAINER FOR INSPECTION 有权
    INSPEKTIONSBEHÄLTER

    公开(公告)号：EP2107008A1

    公开(公告)日：2009-10-07

    申请号：EP08703417.9

    申请日：2008-01-18

    申请人： Toyo Seikan Kaisha, Ltd. ,  EIKEN KAGAKU KABUSHIKI KAISHA

    发明人： SHIBATA, Tomoyuki ,  HOSOKAWA, Manabu ,  HAYASHI, Hiroaki ,  MASUYA, Natsuki ,  SETO, Yoshiaki ,  SAITO, Shingo ,  KANDA, Hidetoshi

    IPC分类号： B65D47/36 ,  B65D51/22 ,  G01N1/10

    CPC分类号： B65D47/36 ,  B01L3/50825 ,  B01L2200/026 ,  B01L2200/141 ,  B01L2300/044 ,  B01L2300/0672 ,  B01L2300/0832 ,  B65D51/222 ,  B65D2251/0025 ,  B65D2251/0087

    摘要： Provided is a container 1 for inspection which stores and pours out contents therein, arid the container is provided with a container main body 2 having a mouth portion 22 formed thereon, a cover portion 3 attached to the mouth portion 22 to seal the container main body 2, and an opening cap 4 attached to the cover portion 3. The cover portion 3 has a closing wall 34 for closing the mouth portion 22, and the opening cap 4 is provided with an pouring outlet 421, and a cutting portion 431 for cutting the closing wall 34. When the opening cap 4 is attached to the cover portion 3, the cutting section 431 cuts the closing wall 34 to enable pouring out the contents.
    Thus, in the provided container 1 for inspection, sealing properties of the container main body 2 are ensured by the cover portion 3, and the closing wall 34 of the cover portion 3 is cut by the opening cap 4 attached to the cover portion 3 without removing the cover portion 3, whereby the contents can be poured out.

    摘要翻译： 设有用于检查的容器1，其中存储并倾倒内容物，并且容器设置有形成有口部22的容器主体2，盖部3，其附接到口部22以密封容器主体 盖部3具有用于封闭口部22的封闭壁34，开口盖4设置有倾倒出口421，切割部431用于切断 封闭壁34.当打开盖4附接到盖部分3上时，切割部分431切割封闭壁34以便能够倾倒内容物。 因此，在所提供的检查容器1中，通过盖部3确保容器主体2的密封性，盖部3的封闭壁34被安装在盖部3上的开口盖4切断而没有 移除盖部分3，从而可以倒出内容物。