发明公开
EP2836503A1 GENETICALLY ENCODED PROBE FOR QUANTIFICATION OF LACTATE, AND METHODS FOR QUANTIFYING METABOLIC RATES AND LACTATE TRANSPORT
有权
用于定量乳酸盐的遗传编码探针，以及用于定量测定代谢速率和乳酸盐运输的方法
- 专利标题： GENETICALLY ENCODED PROBE FOR QUANTIFICATION OF LACTATE, AND METHODS FOR QUANTIFYING METABOLIC RATES AND LACTATE TRANSPORT
- 专利标题（中）： 用于定量乳酸盐的遗传编码探针，以及用于定量测定代谢速率和乳酸盐运输的方法
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申请号： EP12874169.1申请日： 2012-04-13
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公开(公告)号： EP2836503A1公开(公告)日： 2015-02-18
- 发明人: BARROS OLMEDO, Luis Felipe , SAN MARTIN, Alejandro , CEBALLO CHARPENTIER, Sebastian , FROMMER, Wolf, B.
- 申请人： Centro De Estudios Cientificos De Valdivia , Carnegie Institution Of Washington
- 申请人地址： Arturo Prat 514 Valdivia CL
- 专利权人： Centro De Estudios Cientificos De Valdivia,Carnegie Institution Of Washington
- 当前专利权人： Centro De Estudios Cientificos De Valdivia,Carnegie Institution Of Washington
- 当前专利权人地址： Arturo Prat 514 Valdivia CL
- 代理机构： Carvajal y Urquijo, Isabel
- 国际公布： WO2013154587 20131017
- 主分类号： C07K1/00
- IPC分类号： C07K1/00 ; C07H21/04 ; C12N5/00
摘要：
A nanosensor for detecting and quantifying lactate in different types of samples, such as tissues, intra-cellular and subcellular compartments, with high spatial and temporal resolution is disclosed. Methods comprising use of the nanosensor for quantifying the activity of lactate transporters, rates of cellular lactate production and cellular lactate consumption, and rate of mitochondrial pyruvate consumption are also disclosed. Methods for quantifying the transformation in energy metabolism that characterizes cancer cells with single-cell resolution and for detecting interference of candidate drugs with mitochondrial energetics are additionally disclosed.
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