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1.发明授权GENETICALLY ENCODED PROBE FOR QUANTIFICATION OF LACTATE, AND METHODS FOR QUANTIFYING METABOLIC RATES AND LACTATE TRANSPORT 有权Sa基因存储器中的探针用于LACTAT和方法，定量的代谢率与LACTATTRANSPORT定量
公开(公告)号：EP2836503B1
公开(公告)日：2016-06-08
申请号：EP12874169.1
申请日：2012-04-13
发明人： BARROS OLMEDO, Luis Felipe , SAN MARTIN, Alejandro , CEBALLO CHARPENTIER, Sebastian , FROMMER, Wolf, B.
IPC分类号： G01N33/542
CPC分类号： G01N33/5035 , C12N15/85 , G01N33/5038 , G01N33/542 , G01N33/582
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2.发明公开GENETICALLY ENCODED PROBE FOR QUANTIFICATION OF LACTATE, AND METHODS FOR QUANTIFYING METABOLIC RATES AND LACTATE TRANSPORT 有权用于定量乳酸盐的遗传编码探针，以及用于定量测定代谢速率和乳酸盐运输的方法
公开(公告)号：EP2836503A1
公开(公告)日：2015-02-18
申请号：EP12874169.1
申请日：2012-04-13
发明人： BARROS OLMEDO, Luis Felipe , SAN MARTIN, Alejandro , CEBALLO CHARPENTIER, Sebastian , FROMMER, Wolf, B.
CPC分类号： G01N33/5035 , C12N15/85 , G01N33/5038 , G01N33/542 , G01N33/582
摘要： A nanosensor for detecting and quantifying lactate in different types of samples, such as tissues, intra-cellular and subcellular compartments, with high spatial and temporal resolution is disclosed. Methods comprising use of the nanosensor for quantifying the activity of lactate transporters, rates of cellular lactate production and cellular lactate consumption, and rate of mitochondrial pyruvate consumption are also disclosed. Methods for quantifying the transformation in energy metabolism that characterizes cancer cells with single-cell resolution and for detecting interference of candidate drugs with mitochondrial energetics are additionally disclosed.
摘要翻译： 公开了一种纳米传感器，用于以高空间和时间分辨率检测和定量不同类型的样品（例如组织，细胞内和亚细胞区室）中的乳酸盐。 还公开了包括使用纳米传感器来定量乳酸转运蛋白活性，细胞乳酸产生速率和细胞乳酸消耗速率以及线粒体丙酮酸消耗速率的方法。 另外公开了量化表征具有单细胞分辨率的癌细胞和用于检测候选药物与线粒体能量学物质的干扰的能量代谢转化的方法。
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公开(公告)号：EP2427471A2
公开(公告)日：2012-03-14
申请号：EP10772690.3
申请日：2010-05-04
发明人： FROMMER, Wolf, B. , LALONDE, Sylvie
CPC分类号： C12N15/8279 , C07K14/415 , C07K14/43545 , C07K14/47 , C12N15/8218 , C12N15/8245 , C12N15/8246
摘要： A novel class of transporter protein, referred to as SWEET, GLUE or Glü, is disclosed. These transporters provide a novel system for the transportation of sugars across membranes within a cell and between the inside and outside of a cell. Such transporters are useful for understanding and altering the sugar concentration within certain organs of an organism, and within certain organelles within the cell. These transporters are also useful in protecting plants from a pathogen attack.
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公开(公告)号：EP1805200B1
公开(公告)日：2012-01-04
申请号：EP05812420.7
申请日：2005-10-14
CPC分类号： G01N33/542 , G01N33/9406 , G01N2500/00
摘要： Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotranmitter concentrations in vivo and in culture.
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公开(公告)号：EP2181328A1
公开(公告)日：2010-05-05
申请号：EP08795178.6
申请日：2008-08-08
CPC分类号： G01N33/542 , C12Q1/025 , G01N33/5038 , G01N33/66 , G01N2021/6441 , G01N2400/00
摘要： The present invention provides methods for detecting and monitoring metabolite concentrations, which comprise detection and measurement of Fluorescence Resonance Energy Transfer upon ligand binding. The methods of the present invention are useful for real time monitoring of changes in metabolite levels in living cell cultures.
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公开(公告)号：EP1933614A2
公开(公告)日：2008-06-25
申请号：EP05808452.6
申请日：2005-10-14
发明人： FROMMER, Wolf, B. , LAGER, Ida
IPC分类号： A01H1/00
CPC分类号： C07K14/195 , C07K2319/20 , C07K2319/60 , C12Q1/025 , G01N33/5044
摘要： Sucrose biosensors are disclosed, which comprise a sucrose binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon sucrose binding. Such biosensors are useful for real time monitoring of sucrose metabolism in living cells.
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公开(公告)号：EP1933614B1
公开(公告)日：2010-11-24
申请号：EP05808452.6
申请日：2005-10-14
发明人： FROMMER, Wolf, B. , LAGER, Ida
CPC分类号： C07K14/195 , C07K2319/20 , C07K2319/60 , C12Q1/025 , G01N33/5044
摘要： Sucrose biosensors are disclosed, which comprise a sucrose binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon sucrose binding. Such biosensors are useful for real time monitoring of sucrose metabolism in living cells.
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公开(公告)号：EP2971004A1
公开(公告)日：2016-01-20
申请号：EP14773725.8
申请日：2014-03-13
发明人： FROMMER, Wolf, B.
IPC分类号： C12N15/82
CPC分类号： C12N15/8245
摘要： The present invention relates to methods of increasing the levels of at least one sugar in developing seeds in a plant, with the methods comprising inserting an exogenous nucleic acid, which codes for at least one sugar transporter protein (SWEET protein), into a plant cell to create a transgenic plant cell, and subjecting the transgenic plant cell to conditions that promote expression of the at least one SWEET protein during seed development. The methods results in transgenic plant seeds, and transgenic plants that produce seed, where the levels of at least one sugar are increased as compared to seeds from non-transgenic plants of the same species grown under the same conditions.
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公开(公告)号：EP1805200A2
公开(公告)日：2007-07-11
申请号：EP05812420.7
申请日：2005-10-14
CPC分类号： G01N33/542 , G01N33/9406 , G01N2500/00
摘要： Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotranmitter concentrations in vivo and in culture.
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公开(公告)号：EP1948828A2
公开(公告)日：2008-07-30
申请号：EP06837880.1
申请日：2006-11-16
发明人： KAPER, Thijs , FROMMER, Wolf, B.
IPC分类号： C12Q1/68
CPC分类号： C07K14/245 , C07K2319/034 , C07K2319/60 , G01N33/533 , G01N33/542
摘要： Multimeric tryptophan biosensors are disclosed, which comprise tryptophan-binding domains conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon tryptophan binding. Such biosensors are useful for real time monitoring of tryptophan metabolism in living cells.
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