- 专利标题： Pullulanase variants and polynucleotides encoding same
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申请号： US15112096申请日： 2015-01-21
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公开(公告)号： US10030237B2公开(公告)日： 2018-07-24
- 发明人: Tomoko Matsui , Akihiko Yamagishi
- 申请人： NOVOZYMES A/S
- 申请人地址： DK Bagsvaerd
- 专利权人： NOVOZYMES A/S
- 当前专利权人： NOVOZYMES A/S
- 当前专利权人地址： DK Bagsvaerd
- 代理商 David Fazzolare
- 优先权： EP14152110 20140122; EP14195691 20141201
- 国际申请： PCT/EP2015/051145 WO 20150121
- 国际公布： WO2015/110473 WO 20150730
- 主分类号： C12N9/44
- IPC分类号： C12N9/44 ; C12N9/42 ; C12N9/24 ; C12P19/16 ; C12P21/06 ; C12P19/34 ; C12N15/00 ; C12N1/20 ; C12N1/00 ; C07H21/04 ; C07K1/00 ; C12P19/02 ; C12P7/06
摘要：
The present invention relates to pullulanase variants having increased thermoactivity and comprising substitutions of the parent pullulanase at one or more positions corresponding to positions 393, 143, 150, 243, 244, 345, 346, 368, 370, 373, 381, 382, 385, 387, 402, 429, 430, 431, 432, 456, 486, 492, 610, 624, 631, 632, 665 and 699 of the polypeptide of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The variants are mainly derived from pullulanases from Bacillus deramificans, Bacillus acidopullulyticus or hybrid pullulanases.
公开/授权文献
- US20170044511A1 PULLULANASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 公开/授权日：2017-02-16
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