Invention Grant
- Patent Title: Digital counting of individual molecules by stochastic attachment of diverse labels
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Application No.: US15217896Application Date: 2016-07-22
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Publication No.: US10059991B2Publication Date: 2018-08-28
- Inventor: Stephen P. A. Fodor , Glenn K. Fu
- Applicant: Cellular Research, Inc.
- Applicant Address: US CA Menlo Park
- Assignee: CELLULAR RESEARCH, INC.
- Current Assignee: CELLULAR RESEARCH, INC.
- Current Assignee Address: US CA Menlo Park
- Agency: Knobbe Martens Olson & Bear, LLP
- Main IPC: C12N15/10
- IPC: C12N15/10 ; G06F19/20 ; G06F19/22 ; C12Q1/6874 ; C12Q1/6809 ; C12Q1/686 ; C12Q1/6869 ; C12Q1/6837 ; C12Q1/6876 ; C12Q1/6851

Abstract:
Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.
Public/Granted literature
- US20160376583A1 DIGITAL COUNTING OF INDIVIDUAL MOLECULES BY STOCHASTIC ATTACHMENT OF DIVERSE LABELS Public/Granted day:2016-12-29
Information query
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