The present disclosure relates to a thin film transistor substrate having a color filter layer. The present disclosure provides a thin film transistor substrate comprising: a plurality of pixel areas disposed in a matrix manner on a substrate, each pixel area including an aperture area and a non-aperture area; a first color filter and a second color filter stacked at the non-aperture area on the substrate; an overcoat layer disposed on the first color filter and the second color filter; a semiconductor layer disposed at the non-aperture area on the overcoat layer; a gate insulating layer and a gate electrode stacked on a middle portion of the semiconductor layer; a third color filter at the non-aperture area on the semiconductor layer and the gate electrode; and a source electrode and a drain electrode disposed on the third color filter.