Invention Grant
- Patent Title: High molecular weight PHA-producing microbe and method of producing high molecular weight PHA using same
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Application No.: US14437672Application Date: 2013-10-22
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Publication No.: US10233468B2Publication Date: 2019-03-19
- Inventor: Hisashi Arikawa , Shunsuke Sato , Keiji Matsumoto
- Applicant: KANEKA CORPORATION
- Applicant Address: JP Kita-ku
- Assignee: KANEKA CORPORATION
- Current Assignee: KANEKA CORPORATION
- Current Assignee Address: JP Kita-ku
- Agency: Oblon, McClelland, Maier & Neustadt, L.L.P.
- Priority: JP2012-232694 20121022; JP2012-232695 20121022
- International Application: PCT/JP2013/078520 WO 20131022
- International Announcement: WO2014/065253 WO 20140501
- Main IPC: C12P7/62
- IPC: C12P7/62 ; C12N15/74 ; C12N9/18

Abstract:
An object of the present invention is to provide a microorganism strain that accumulates a high molecular weight PHA, and a PHA production method using the microorganism. The present invention provides a method for producing a PHA copolymer, which includes culturing a microorganism, wherein at least a portion of either of the following genes (a) and (b) of the microorganism has been altered by substitution, deletion, insertion, and/or addition to reduce or eliminate the activity of a PHA degrading enzyme encoded by the gene: (a) a PHA degrading enzyme gene encoding the amino acid sequence of SEQ ID NO:2 in the sequence listing; and (b) a gene encoding a polypeptide having at least 85% sequence identity to the amino acid sequence of SEQ ID NO:2 in the sequence listing and having PHA degrading enzyme activity.
Public/Granted literature
- US20160237462A1 HIGH MOLECULAR WEIGHT PHA-PRODUCING MICROBE AND METHOD OF PRODUCING HIGH MOLECULAR WEIGHT PHA USING SAME Public/Granted day:2016-08-18
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