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公开(公告)号：US10233468B2
公开(公告)日：2019-03-19
申请号：US14437672
申请日：2013-10-22
Applicant: KANEKA CORPORATION
Inventor： Hisashi Arikawa , Shunsuke Sato , Keiji Matsumoto
Abstract: An object of the present invention is to provide a microorganism strain that accumulates a high molecular weight PHA, and a PHA production method using the microorganism. The present invention provides a method for producing a PHA copolymer, which includes culturing a microorganism, wherein at least a portion of either of the following genes (a) and (b) of the microorganism has been altered by substitution, deletion, insertion, and/or addition to reduce or eliminate the activity of a PHA degrading enzyme encoded by the gene: (a) a PHA degrading enzyme gene encoding the amino acid sequence of SEQ ID NO:2 in the sequence listing; and (b) a gene encoding a polypeptide having at least 85% sequence identity to the amino acid sequence of SEQ ID NO:2 in the sequence listing and having PHA degrading enzyme activity.
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公开(公告)号：US12286662B2
公开(公告)日：2025-04-29
申请号：US17763886
申请日：2020-08-05
Applicant: KANEKA CORPORATION
Inventor： Hisashi Arikawa , Shunsuke Sato , Yoshihiro Mouri
IPC: C12P7/625
Abstract: A polyhydroxyalkanoate-producing microorganism is cultured to obtain microbial bodies accumulating polyhydroxyalkanoate particles and having an average cell size of 2 μm or more. The microbial bodies are subjected to a heat treatment at a temperature higher than a temperature in the culturing to increase an average particle size of the polyhydroxyalkanoate particles in the microbial bodies. The resulting average particle size is equal to or greater than 1.8 μm and equal to or smaller than the average cell size. The microbial bodies subjected to the heat treatment can be disrupted to obtain a cell disruption solution. The PHA particles can be separated from an aqueous phase of the cell disruption solution.
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公开(公告)号：US11186831B2
公开(公告)日：2021-11-30
申请号：US16962282
申请日：2019-01-10
Applicant: KANEKA CORPORATION
Inventor： Shingo Kobayashi , Shinichi Yoshida , Shunsuke Sato , Naoaki Taoka
Abstract: Provided is a mutant PHA synthase which produces of a PHA copolymer with a high or low 3HH ratio while maintaining PHA productivity. The mutant PHA synthase is a mutant polyhydroxyalkanoate synthase having an amino acid sequence having 85% or more sequence identity with the amino acid sequence of SEQ ID NO: 1 and having at least one of the following mutations (a) to (c): (a) a substitution of serine at 389th position from N-terminus of the amino acid sequence of SEQ ID NO: 1 with an amino acid other than serine; (b) a substitution of leucine at 436th position from the N-terminus of the amino acid sequence of SEQ ID NO: 1 with an amino acid other than leucine; and (c) a deletion of 11 to 19 amino acid residues from the C-terminus of the amino acid sequence of SEQ ID NO: 1.
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公开(公告)号：US12240955B2
公开(公告)日：2025-03-04
申请号：US17627356
申请日：2020-07-10
Applicant: KANEKA CORPORATION
Inventor： Asuka Fukutome , Tetsuo Okura , Shunsuke Sato
Abstract: A method for producing a poly(3-hydroxybutyrate) resin-containing composition for melt processing includes: heating a material containing a poly(3-hydroxybutyrate) resin to a temperature equal to or higher than a melting point peak temperature in differential scanning calorimetry analysis of the poly(3-hydroxybutyrate) resin and equal to or lower than a melting point peak end temperature in the differential scanning calorimetry analysis of the poly(3-hydroxybutyrate) resin, wherein the difference between the melting point peak temperature and the melting point peak end temperature of the poly(3-hydroxybutyrate) resin is 10° C. or more; and extruding the heated material to obtain a composition for melt processing that has a new crystallization peak at a temperature higher than the melting point peak temperature.
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公开(公告)号：US12134217B2
公开(公告)日：2024-11-05
申请号：US17627428
申请日：2020-06-08
Applicant: KANEKA CORPORATION
Inventor： Yuta Katsuta , Nobuo Nakamura , Shunsuke Sato
IPC: B29C45/00 , C08G63/06 , C08K3/34 , B29C45/72 , B29K105/00 , B29K509/02
Abstract: A method for producing an injection-molded body from a molding material containing a poly(3-hydroxybutyrate) resin includes: melting the molding material by heating the molding material to a temperature between a melting point peak temperature and a melting point peak end temperature in differential scanning calorimetry analysis of the molding material, wherein the difference between the melting point peak temperature and the melting point peak end temperature of the molding material is from 10 to 70° C.; injecting the molten molding material into a mold having a temperature of 30 to 80° C.; and cooling the molding material in the mold to crystallize and solidify the molding material.
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Patent Agency Ranking
公开(公告)号：US11634696B2
公开(公告)日：2023-04-25
申请号：US17252086
申请日：2019-06-12
Applicant: KANEKA CORPORATION
Inventor： Shingo Kobayashi , Shunsuke Sato , Naoaki Taoka
Abstract: Provided is a mutant polyhydroxyalkanoate synthetase having an amino acid sequence which has 85% or more sequence identity with the amino acid sequence of SEQ ID NO: 1 and which contains a mutation of substitution of at least one of the amino acids at the 27th to 33rd, 39th, 56th, 106th, 129th, 144th, 165th, 170th and 172nd to 187th positions from the N-terminus of the amino acid sequence of SEQ ID NO: 1 with another amino acid.
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公开(公告)号：US20230265468A1
公开(公告)日：2023-08-24
申请号：US18133912
申请日：2023-04-12
Applicant: KANEKA CORPORATION
Inventor： Ken Harada , Shunsuke Sato
IPC: C12P7/625
CPC classification number: C12P7/625
Abstract: In culturing a poly(3-hydroxyalkanoate)-producing microorganism, an oil A and an oil B are used as the carbon source. The proportion of the oil B to the total amount of the oils A and B used during the entire course of the culturing is 10 wt% or more. The oil A is the total oil used until the amount of the poly(3-hydroxyalkanoate) accumulated in the poly(3-hydroxyalkanoate)-producing microorganism reaches 16 wt%, and the average unsaturated fatty acid content in constituent fatty acids is from 25 wt% to less than 75 wt% in the total oil A. The unsaturated fatty acid content in constituent fatty acids in the oil B is higher than the average unsaturated fatty acid content in the total oil A.
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公开(公告)号：US11268113B2
公开(公告)日：2022-03-08
申请号：US16616276
申请日：2018-05-23
Applicant: KANEKA CORPORATION
Inventor： Rina Aoki , Shunsuke Sato
Abstract: Provided are a PHA-producing microorganism producing a higher molecular weight PHA and a PHA production method using the PHA-producing microorganism. A PHA-producing microorganism including a gene encoding a PHA synthase derived from genus Aeromonas, in which at least a portion of a PHA degrading enzyme gene is altered by substitution, deletion, insertion, and/or addition to reduce or eliminate activity of a PHA degrading enzyme encoded by the gene, and further a glycerol kinase activity is enhanced.
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公开(公告)号：US20200340020A1
公开(公告)日：2020-10-29
申请号：US16927229
申请日：2020-07-13
Applicant: KANEKA CORPORATION
Inventor： Hisashi ARIKAWA , Shunsuke Sato
Abstract: The present application provides: a transformed microorganism for producing a PHA copolymer containing 3HH monomer unit at a higher composition ratio, specifically, a transformed microorganism comprising a PHA synthase gene capable of synthesizing a PHA copolymer containing 3HH monomer unit and a gene encoding a protein having (R)-specific enoyl-CoA hydratase activity, characterized in that, in the transformed microorganism, the expression of a gene encoding at least one β-ketothiolase enzyme having thiolysis activity for β-keto-(C6) acyl-CoA (i.e., β-ketohexanoyl-CoA) is inhibited, thereby losing or reducing the enzyme activity; and a method for producing a PHA copolymer containing 3HH monomer unit, comprising a step of culturing the transformed microorganism.
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公开(公告)号：US10072255B2
公开(公告)日：2018-09-11
申请号：US15115394
申请日：2015-01-30
Applicant: KANEKA CORPORATION
Inventor： Hisashi Arikawa , Shunsuke Sato , Keiji Matsumoto
CPC classification number: C12N9/88 , C12N15/74 , C12P7/625 , C12Y402/01017 , C12Y402/01074
Abstract: This invention relates to a microorganism that produces a polyhydroxyalkanoate (PHA) copolymer with a regulated monomer composition ratio and comprises a (R)-specific enoyl-CoA hydratase gene in the genome DNA, wherein a nucleotide sequence upstream of the (R)-specific enoyl-CoA hydratase gene comprises a modification consisting of a substitution(s), a deletion(s), an insertion(s), and/or an addition(s) of one or a plurality of nucleotides so that the expression of the (R)-specific enoyl-CoA hydratase gene is regulated, and to a method for producing a PHA copolymer using the microorganism.
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