Invention Grant
- Patent Title: Method of making a device for generating droplets
-
Application No.: US15365888Application Date: 2016-11-30
-
Publication No.: US10258989B2Publication Date: 2019-04-16
- Inventor: Kevin D. Ness , Benjamin J. Hindson , Billy W. Colston, Jr. , Donald A. Masquelier
- Applicant: Bio-Rad Laboratories, Inc.
- Applicant Address: US CA Hercules
- Assignee: Bio-Rad Laboratories, Inc.
- Current Assignee: Bio-Rad Laboratories, Inc.
- Current Assignee Address: US CA Hercules
- Agency: Kolisch Hartwell, P.C.
- Main IPC: B01L3/00
- IPC: B01L3/00 ; B01F3/08 ; B01F13/00 ; B01L3/02 ; B01L7/00 ; G01N21/3563 ; C12Q1/686 ; G01N21/64 ; B01F15/00 ; B29C45/00 ; G01N21/49 ; B29L31/00

Abstract:
Methods of making a droplet-generating device. In an exemplary method, an upper member is injection molded. The upper member includes a bottom surface and also includes a first microfluidic channel, a second microfluidic channel, and a third microfluidic channel each formed in the bottom surface. The upper member has a plurality of openings each extending completely through the upper member from the bottom surface and creating a side wall region of a sample well, a carrier well, and a droplet well. A cover layer is attached to the bottom surface of the upper member, such that the cover layer seals a bottom side of each microfluidic channel. The microfluidic channels meet one another to create a droplet-generation region. The sample well, the carrier well, and the droplet well are connected to the droplet-generation region via the first, second, and third microfluidic channels, respectively.
Public/Granted literature
- US20170144116A1 METHOD OF MAKING A DEVICE FOR GENERATING DROPLETS Public/Granted day:2017-05-25
Information query
IPC分类: