A read and write control circuit for a flash chip is disclosed which includes a timing control circuit for generating a read and write timing signal for the flash chip, and a first non-volatile memory for storing a plurality of flags corresponding to a plurality of blocks in the flash chip, each of the flags indicating whether a respective one of the blocks that corresponds thereto has been written to normally. Also disclosed is a read and write control method of a flash chip, as well as an AMOLED application circuit having the read and write control circuit for use in an electrical compensation mechanism.