A method of operating a laboratory sample distribution system is disclosed. The laboratory sample distribution system comprises a plurality of sample container carriers. The sample container carriers carry one or more sample containers. The sample containers comprise samples to be analyzed by a plurality of laboratory stations. The system also comprises a transport plane. The transport plane supports the sample container carriers. The transport plane comprises a plurality of transfer locations. The transfer locations are assigned to corresponding laboratory stations. The system also comprises a drive. The drive moves the sample container carriers on the transport plane. The method comprises, during an initialization of the laboratory sample distribution system, pre-calculating routes depending on the transfer locations and, after the initialization of the laboratory sample distribution system, controlling the drive such that the sample container carriers move along the pre-calculated routes.