- 专利标题: Production of fungal extracellular immune stimulating compounds
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申请号: US15008891申请日: 2016-01-28
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公开(公告)号: US10471135B2公开(公告)日: 2019-11-12
- 发明人: Bjoern Kristiansen
- 申请人: Glycanova AS
- 申请人地址: NO Gamle Fredrikstad
- 专利权人: Glycanova AS
- 当前专利权人: Glycanova AS
- 当前专利权人地址: NO Gamle Fredrikstad
- 代理机构: Merchant & Gould P.C.
- 优先权: NO20014256 20010903
- 主分类号: A61K39/00
- IPC分类号: A61K39/00 ; A61K31/716 ; A61K36/07 ; C12P19/04 ; A61K31/7004
摘要:
A process is described for the production of an immunostimulant by submerged cultivation of Ganoderma lucidum in which mycelium from agar plates or a fermentation broth is added to a liquid medium in a shake flask or a bioreactor containing nutrients such as malt extract, yeast extract, peptone and glucose having access to air or to which air is added, and which is kept in constant movement at approx. 28° C. At the proper conditions, there will be an increase in the production of extracellular lentinan, which is shown to be a better immunostimulant than intracellular lentinan. The extracellular product is precipitated from the growth medium by means of methods for the precipitation of microbial polysaccharide.