公开(公告)号：US09249438B2

公开(公告)日：2016-02-02

申请号：US14162272

申请日：2014-01-23

申请人： Glycanova AS

发明人： Bjoern Kristiansen

IPC分类号： C12P19/04 ,  A61K36/07

CPC分类号： A61K39/0002 ,  A61K31/7004 ,  A61K31/716 ,  A61K36/07 ,  A61K2039/58 ,  C12P19/04 ,  Y02A50/463 ,  Y02A50/465 ,  Y02A50/471 ,  Y02A50/473 ,  Y02A50/481

摘要： A process is described for the production of an immunostimulant by submerged cultivation of Ganoderma lucidum in which mycelium from agar plates or a fermentation broth is added to a liquid medium in a shake flask or a bioreactor containing nutrients such as malt extract, yeast extract, peptone and glucose having access to air or to which air is added, and which is kept in constant movement at approx. 28° C. At the proper conditions, there will be an increase in the production of extracellular lentinan, which is shown to be a better immunostimulant than intracellular lentinan. The extracellular product is precipitated from the growth medium by means of methods for the precipitation of microbial polysaccharide.

摘要翻译： 描述了通过浸没培养灵芝制备免疫刺激剂的方法，其中将来自琼脂平板或发酵液的菌丝体加入摇瓶中的液体培养基或含有营养物质的生物反应器如麦芽提取物，酵母提取物，蛋白胨 和葡萄糖可以进入空气或添加空气，并且在约 28℃。在适当的条件下，细胞外香菇多糖的产生将增加，这显示比细胞内香菇多糖更好的免疫刺激剂。 通过沉淀微生物多糖的方法从生长培养基中沉淀出细胞外产物。

公开(公告)号：US10471135B2

公开(公告)日：2019-11-12

申请号：US15008891

申请日：2016-01-28

申请人： Glycanova AS

发明人： Bjoern Kristiansen

IPC分类号： A61K39/00 ,  A61K31/716 ,  A61K36/07 ,  C12P19/04 ,  A61K31/7004

摘要： A process is described for the production of an immunostimulant by submerged cultivation of Ganoderma lucidum in which mycelium from agar plates or a fermentation broth is added to a liquid medium in a shake flask or a bioreactor containing nutrients such as malt extract, yeast extract, peptone and glucose having access to air or to which air is added, and which is kept in constant movement at approx. 28° C. At the proper conditions, there will be an increase in the production of extracellular lentinan, which is shown to be a better immunostimulant than intracellular lentinan. The extracellular product is precipitated from the growth medium by means of methods for the precipitation of microbial polysaccharide.

公开(公告)号：US20160022786A1

公开(公告)日：2016-01-28

申请号：US14705346

申请日：2015-05-06

申请人： Glycanova AS

发明人： Bjørn Kristiansen

IPC分类号： A61K39/00 ,  A61K45/06

CPC分类号： A61K39/0002 ,  A61K31/70 ,  A61K45/06 ,  A61K2039/585 ,  A61K2300/00

摘要： In one aspect the present invention relates to pharmaceutical kits of parts suitable for treating neoplastic diseases such as cancer comprising an anti-cancer medicament, a Basidiomycete bioactive agent in solid or liquid form, and, optionally instructions for a dosing regime.

摘要翻译： 一方面，本发明涉及适用于治疗肿瘤疾病如癌症的部件的药物试剂盒，其包含抗癌药物，固体或液体形式的担子菌生物活性剂，以及任选的给药方案说明书。

公开(公告)号：US20140186900A1

公开(公告)日：2014-07-03

申请号：US14162272

申请日：2014-01-23

申请人： Glycanova AS

发明人： Bjoern Kristiansen

IPC分类号： C12P19/04

CPC分类号： A61K39/0002 ,  A61K31/7004 ,  A61K31/716 ,  A61K36/07 ,  A61K2039/58 ,  C12P19/04 ,  Y02A50/463 ,  Y02A50/465 ,  Y02A50/471 ,  Y02A50/473 ,  Y02A50/481

摘要： A process is described for the production of an immunostimulant by submerged cultivation of Ganoderma lucidum in which mycelium from agar plates or a fermentation broth is added to a liquid medium in a shake flask or a bioreactor containing nutrients such as malt extract, yeast extract, peptone and glucose having access to air or to which air is added, and which is kept in constant movement at approx. 28° C. At the proper conditions, there will be an increase in the production of extracellular lentinan, which is shown to be a better immunostimulant than intracellular lentinan. The extracellular product is precipitated from the growth medium by means of methods for the precipitation of microbial polysaccharide.

摘要翻译： 描述了通过浸没培养灵芝制备免疫刺激剂的方法，其中将来自琼脂平板或发酵液的菌丝体加入摇瓶中的液体培养基或含有营养物质的生物反应器如麦芽提取物，酵母提取物，蛋白胨 和葡萄糖可以进入空气或添加空气，并且在约 28℃。在适当的条件下，细胞外香菇多糖的产生将增加，这显示比细胞内香菇多糖更好的免疫刺激剂。 通过沉淀微生物多糖的方法从生长培养基中沉淀出细胞外产物。

公开(公告)号：US10729714B2

公开(公告)日：2020-08-04

申请号：US14766811

申请日：2014-02-10

申请人： Glycanova AS

发明人： Bjørn Kristiansen ,  Anne Torill Hovland

IPC分类号： A61K8/99 ,  A61K31/716 ,  A61K36/07 ,  A61K31/728 ,  A61K9/00 ,  A61K9/06 ,  A61K35/644 ,  A61K47/06 ,  A61K47/10 ,  A61K47/12 ,  A61K47/26 ,  A61K47/44 ,  A61K9/70

摘要： An extract of a liquid culture of Ganoderma lucidum is provided. The production process encourages the growing mycelium to export bioactive compounds such as beta glucans into the surrounding liquid. Thus, the beta glucan is soluble and easily absorbed. More importantly, the production process allows the beta glucan to retain its triple helix structure which is required to maintain a very high bioactive efficacy. The beta glucans from Ganoderma lucidum can be used in a cream for treatment of psoriasis.

公开(公告)号：US20160144008A1

公开(公告)日：2016-05-26

申请号：US15008891

申请日：2016-01-28

申请人： Glycanova AS

发明人： Bjoern Kristiansen

IPC分类号： A61K39/00 ,  A61K31/7004 ,  A61K36/07 ,  A61K31/716

CPC分类号： A61K39/0002 ,  A61K31/7004 ,  A61K31/716 ,  A61K36/07 ,  A61K2039/58 ,  C12P19/04 ,  Y02A50/463 ,  Y02A50/465 ,  Y02A50/471 ,  Y02A50/473 ,  Y02A50/481

摘要： A process is described for the production of an immunostimulant by submerged cultivation of Ganoderma lucidum in which mycelium from agar plates or a fermentation broth is added to a liquid medium in a shake flask or a bioreactor containing nutrients such as malt extract, yeast extract, peptone and glucose having access to air or to which air is added, and which is kept in constant movement at approx. 28° C. At the proper conditions, there will be an increase in the production of extracellular lentinan, which is shown to be a better immunostimulant than intracellular lentinan. The extracellular product is precipitated from the growth medium by means of methods for the precipitation of microbial polysaccharide.

摘要翻译： 描述了通过浸没培养灵芝制备免疫刺激剂的方法，其中将来自琼脂平板或发酵液的菌丝体加入摇瓶中的液体培养基或含有营养物质的生物反应器如麦芽提取物，酵母提取物，蛋白胨 和葡萄糖可以进入空气或添加空气，并且在约 28℃。在适当的条件下，细胞外香菇多糖的产生将增加，这显示比细胞内香菇多糖更好的免疫刺激剂。 通过沉淀微生物多糖的方法从生长培养基中沉淀出细胞外产物。

公开(公告)号：US20170304415A1

公开(公告)日：2017-10-26

申请号：US15649288

申请日：2017-07-13

申请人： Glycanova AS

发明人： Bjørn Kristiansen

IPC分类号： A61K39/00 ,  A61K31/70 ,  A61K45/06

CPC分类号： A61K39/0002 ,  A61K31/70 ,  A61K45/06 ,  A61K2039/585 ,  A61K2300/00

摘要： In one aspect the present invention relates to pharmaceutical kits of parts suitable for treating neoplastic diseases such as cancer comprising an anti-cancer medicament, a Basidiomycete bioactive agent in solid or liquid form, and, optionally instructions for a dosing regime.

公开(公告)号：US20160015734A1

公开(公告)日：2016-01-21

申请号：US14766811

申请日：2014-02-10

申请人： GLYCANOVA AS

发明人： Bjørn KRISTIANSEN ,  Anne Torill HOVLAND

IPC分类号： A61K31/716 ,  A61K47/44 ,  A61K47/06 ,  A61K47/10 ,  A61K9/00 ,  A61K35/644 ,  A61K47/26 ,  A61K31/728 ,  A61K9/06 ,  A61K36/07 ,  A61K47/12

摘要： The product according to the present invention is produced in a production process using the mycelium of a mushroom such as Ganoderma lucidum. The production process encourages the growing mycelium to export bioactive compounds such as beta glucans into the surrounding liquid. Thus, the beta glucan is soluble and easily absorbed. More importantly, the production process allows the beta glucan to retain its triple helix structure which is required for maintain a very high bioactive efficacy. The beta glucans from Ganoderma lucidum can be used in a cream for treatment of psoriasis.

摘要翻译： 根据本发明的产品在使用蘑菇菌丝体如灵芝的生产过程中生产。 生产过程鼓励生长的菌丝体将生物活性化合物如β-葡聚糖引入周围的液体中。 因此，β葡聚糖是可溶的并容易吸收。 更重要的是，生产过程允许β-葡聚糖保留其维持非常高的生物活性效力所需的三螺旋结构。 来自灵芝的β葡聚糖可用于治疗牛皮癣的霜剂。