Invention Grant
- Patent Title: Methods and kits for post-IVT RNA purification
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Application No.: US16444211Application Date: 2019-06-18
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Publication No.: US10731147B2Publication Date: 2020-08-04
- Inventor: Yi-Cheng Hsieh , Cheng-I Hsu , Chia-Ling Wu , Hsan Jan Yen
- Applicant: CORNING INCORPORATED
- Applicant Address: US NY Corning
- Assignee: Corning Incorporated
- Current Assignee: Corning Incorporated
- Current Assignee Address: US NY Corning
- Agent F. Brock Riggs
- Main IPC: C12N15/10
- IPC: C12N15/10

Abstract:
The disclosure relates to methods for nucleic acid purification, comprising (a) combining a sample comprising at least one nucleic acid with a binding buffer comprising at least one magnetic particle and having a pH ranging from about 4 to about 10 to form a solution; (b) incubating the sample with the binding buffer for a time period sufficient to reversibly bind the at least one nucleic acid to the at least one magnetic particle to form at least one modified magnetic particle, (c) separating the at least one modified magnetic particle from the solution, (d) washing the at least one modified magnetic particle with at least one wash buffer; and (e) combining the at least one modified magnetic particle with an elution buffer Kits comprising these buffers and magnetic particles are also disclosed herein.
Public/Granted literature
- US20190300874A1 METHODS AND KITS FOR POST-IVT RNA PURIFICATION Public/Granted day:2019-10-03
Information query
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