公开(公告)号：US20170362586A1

公开(公告)日：2017-12-21

申请号：US15533547

申请日：2015-12-09

Applicant: Corning Incorporated

Inventor： Yi-Cheng Hsieh ,  Cheng-l Hsu ,  Jun Min Hu ,  Chia-Ling Wu ,  Hsan Jan Yen

IPC: C12N15/10

CPC classification number: C12N15/1013

Abstract: The disclosure relates to methods for nucleic acid purification, comprising (a) combining a sample comprising at least one nucleic acid with a suspension buffer to form a suspension, (b) combining the suspension with a lysis buffer to form a lysate, (c) combining the lysate with a binding buffer to form a solution; (d) combining the solution with at least one magnetic particle to form a combined solution comprising at least one modified magnetic particle reversibly bound to the at least one nucleic acid, (e) separating the at least one modified magnetic particle from the combined solution, (f) washing the at least one modified magnetic particle with a first wash buffer and a second wash buffer, and (g) combining the at least one modified magnetic particle with an elution buffer. Kits comprising these buffers and magnetic particles are also disclosed herein.

公开(公告)号：US10364428B2

公开(公告)日：2019-07-30

申请号：US15526651

申请日：2015-11-10

Applicant: Corning Incorporated

Inventor： Yi-Cheng Hsieh ,  Cheng-I Hsu ,  Chia-Ling Wu ,  Hsan-Jan Yen

IPC: C12N15/10

Abstract: The disclosure relates to methods for nucleic acid purification, comprising (a) combining a sample comprising at least one nucleic acid with a binding buffer comprising at least one magnetic particle and having a pH ranging from about 4 to about 10 to form a solution; (b) incubating the sample with the binding buffer for a time period sufficient to reversibly bind the at least one nucleic acid to the at least one magnetic particle to form at least one modified magnetic particle, (c) separating the at least one modified magnetic particle from the solution, (d) washing the at least one modified magnetic particle with at least one wash buffer; and (e) combining the at least one modified magnetic particle with an elution buffer Kits comprising these buffers and magnetic particles are also disclosed herein.

公开(公告)号：US20170314010A1

公开(公告)日：2017-11-02

申请号：US15526651

申请日：2015-11-10

Applicant: Corning Incorporated

Inventor： Yi-Cheng Hsieh ,  Cheng-I Hsu ,  Chia-Ling Wu ,  Hsan-Jan Yen

IPC: C12N15/10

CPC classification number: C12N15/1013

Abstract: The disclosure relates to methods for nucleic acid purification, comprising (a) combining a sample comprising at least one nucleic acid with a binding buffer comprising at least one magnetic particle and having a pH ranging from about 4 to about 10 to form a solution; (b) incubating the sample with the binding buffer for a time period sufficient to reversibly bind the at least one nucleic acid to the at least one magnetic particle to form at least one modified magnetic particle, (c) separating the at least one modified magnetic particle from the solution, (d) washing the at least one modified magnetic particle with at least one wash buffer; and (e) combining the at least one modified magnetic particle with an elution buffer Kits comprising these buffers and magnetic particles are also disclosed herein.

公开(公告)号：US20190300874A1

公开(公告)日：2019-10-03

申请号：US16444211

申请日：2019-06-18

Applicant: CORNING INCORPORATED

Inventor： Yi-Cheng Hsieh ,  Cheng-I Hsu ,  Chia-Ling Wu ,  Hsan Jan Yen

IPC: C12N15/10

Abstract: The disclosure relates to methods for nucleic acid purification, comprising (a) combining a sample comprising at least one nucleic acid with a binding buffer comprising at least one magnetic particle and having a pH ranging from about 4 to about 10 to form a solution; (b) incubating the sample with the binding buffer for a time period sufficient to reversibly bind the at least one nucleic acid to the at least one magnetic particle to form at least one modified magnetic particle, (c) separating the at least one modified magnetic particle from the solution, (d) washing the at least one modified magnetic particle with at least one wash buffer; and (e) combining the at least one modified magnetic particle with an elution buffer Kits comprising these buffers and magnetic particles are also disclosed herein.

公开(公告)号：US10731147B2

公开(公告)日：2020-08-04

申请号：US16444211

申请日：2019-06-18

Applicant: CORNING INCORPORATED

Inventor： Yi-Cheng Hsieh ,  Cheng-I Hsu ,  Chia-Ling Wu ,  Hsan Jan Yen

IPC: C12N15/10

Abstract: The disclosure relates to methods for nucleic acid purification, comprising (a) combining a sample comprising at least one nucleic acid with a binding buffer comprising at least one magnetic particle and having a pH ranging from about 4 to about 10 to form a solution; (b) incubating the sample with the binding buffer for a time period sufficient to reversibly bind the at least one nucleic acid to the at least one magnetic particle to form at least one modified magnetic particle, (c) separating the at least one modified magnetic particle from the solution, (d) washing the at least one modified magnetic particle with at least one wash buffer; and (e) combining the at least one modified magnetic particle with an elution buffer Kits comprising these buffers and magnetic particles are also disclosed herein.

公开(公告)号：US20180355346A1

公开(公告)日：2018-12-13

申请号：US15757830

申请日：2016-09-09

Applicant: Corning Incorporated

Inventor： Yi-Cheng Hsieh ,  Cheng-l Hsu ,  Chia-Ling Wu ,  Hsan Jan Yen

IPC: C12N15/10 ,  C12Q1/6806

CPC classification number: C12N15/1013 ,  C12N15/1006 ,  C12Q1/6806

Abstract: Provided herein are systems, kits, and methods for nucleic acid purification from blood samples. In particular, provided herein are reagents, e.g. dissolving buffer comprising sarcosine, and methods of using such reagents, for high yield purification of nucleic acids from blood samples, including dried blood samples. Also claimed is a system for purifying nucleic acids from whole blood samples comprising a lysis buffer comprising guanidine and disodium hydrogen phosphate. Magnetic particles are suggested as solid support for capturing the nucleic acids.