公开(公告)号：US10731147B2

公开(公告)日：2020-08-04

申请号：US16444211

申请日：2019-06-18

Applicant: CORNING INCORPORATED

Inventor： Yi-Cheng Hsieh ,  Cheng-I Hsu ,  Chia-Ling Wu ,  Hsan Jan Yen

IPC: C12N15/10

Abstract: The disclosure relates to methods for nucleic acid purification, comprising (a) combining a sample comprising at least one nucleic acid with a binding buffer comprising at least one magnetic particle and having a pH ranging from about 4 to about 10 to form a solution; (b) incubating the sample with the binding buffer for a time period sufficient to reversibly bind the at least one nucleic acid to the at least one magnetic particle to form at least one modified magnetic particle, (c) separating the at least one modified magnetic particle from the solution, (d) washing the at least one modified magnetic particle with at least one wash buffer; and (e) combining the at least one modified magnetic particle with an elution buffer Kits comprising these buffers and magnetic particles are also disclosed herein.

公开(公告)号：US10364428B2

公开(公告)日：2019-07-30

申请号：US15526651

申请日：2015-11-10

Applicant: Corning Incorporated

Inventor： Yi-Cheng Hsieh ,  Cheng-I Hsu ,  Chia-Ling Wu ,  Hsan-Jan Yen

IPC: C12N15/10

Abstract: The disclosure relates to methods for nucleic acid purification, comprising (a) combining a sample comprising at least one nucleic acid with a binding buffer comprising at least one magnetic particle and having a pH ranging from about 4 to about 10 to form a solution; (b) incubating the sample with the binding buffer for a time period sufficient to reversibly bind the at least one nucleic acid to the at least one magnetic particle to form at least one modified magnetic particle, (c) separating the at least one modified magnetic particle from the solution, (d) washing the at least one modified magnetic particle with at least one wash buffer; and (e) combining the at least one modified magnetic particle with an elution buffer Kits comprising these buffers and magnetic particles are also disclosed herein.

公开(公告)号：US20170314010A1

公开(公告)日：2017-11-02

申请号：US15526651

申请日：2015-11-10

Applicant: Corning Incorporated

Inventor： Yi-Cheng Hsieh ,  Cheng-I Hsu ,  Chia-Ling Wu ,  Hsan-Jan Yen

IPC: C12N15/10

CPC classification number: C12N15/1013

Abstract: The disclosure relates to methods for nucleic acid purification, comprising (a) combining a sample comprising at least one nucleic acid with a binding buffer comprising at least one magnetic particle and having a pH ranging from about 4 to about 10 to form a solution; (b) incubating the sample with the binding buffer for a time period sufficient to reversibly bind the at least one nucleic acid to the at least one magnetic particle to form at least one modified magnetic particle, (c) separating the at least one modified magnetic particle from the solution, (d) washing the at least one modified magnetic particle with at least one wash buffer; and (e) combining the at least one modified magnetic particle with an elution buffer Kits comprising these buffers and magnetic particles are also disclosed herein.

公开(公告)号：US20230045516A1

公开(公告)日：2023-02-09

申请号：US17782379

申请日：2020-12-09

Applicant: CORNING INCORPORATED

Inventor： Pei-Chen Chiang ,  Qiang Fu ,  Cheng-I Hsu ,  Wei-Lun Hsu

IPC: C12N15/87 ,  C03C12/00 ,  C03C17/28 ,  C03C3/064 ,  C03C3/097 ,  C03C3/112 ,  C03C4/00 ,  C03C8/06 ,  C03C8/08 ,  C03C8/16

Abstract: A pH-switchable carrier composition includes a plurality of bioactive glass particles, wherein each of the bioactive glass particle is optionally at least a partially coated with a surface modifier; wherein the bioactive glass particles, with or without, the surface modifier can bind to a nucleic acid compound upon contact at pH in the range of about 7 to about 11, and exhibit controlled release of the nucleic acid compound at pH in the range of about 5 to 6.

公开(公告)号：US20190300874A1

公开(公告)日：2019-10-03

申请号：US16444211

申请日：2019-06-18

Applicant: CORNING INCORPORATED

Inventor： Yi-Cheng Hsieh ,  Cheng-I Hsu ,  Chia-Ling Wu ,  Hsan Jan Yen

IPC: C12N15/10

Abstract: The disclosure relates to methods for nucleic acid purification, comprising (a) combining a sample comprising at least one nucleic acid with a binding buffer comprising at least one magnetic particle and having a pH ranging from about 4 to about 10 to form a solution; (b) incubating the sample with the binding buffer for a time period sufficient to reversibly bind the at least one nucleic acid to the at least one magnetic particle to form at least one modified magnetic particle, (c) separating the at least one modified magnetic particle from the solution, (d) washing the at least one modified magnetic particle with at least one wash buffer; and (e) combining the at least one modified magnetic particle with an elution buffer Kits comprising these buffers and magnetic particles are also disclosed herein.