Invention Grant
- Patent Title: Kaurenoic acid hydroxylases
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Application No.: US15765549Application Date: 2016-10-05
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Publication No.: US10760085B2Publication Date: 2020-09-01
- Inventor: Johannes Gustaaf Ernst Van Leeuwen , Priscilla Zwartjens , Viktor Marius Boer
- Applicant: DSM IP ASSETS B.V.
- Applicant Address: NL Heerlen
- Assignee: DSM IP ASSETS B.V.
- Current Assignee: DSM IP ASSETS B.V.
- Current Assignee Address: NL Heerlen
- Agency: McBee Moore Woodward & Vanik IP, LLC
- Priority: com.zzzhc.datahub.patent.etl.us.BibliographicData$PriorityClaim@5eb69272
- International Application: PCT/EP2016/073818 WO 20161005
- International Announcement: WO2017/060318 WO 20170413
- Main IPC: C12N15/81
- IPC: C12N15/81 ; C12N9/02 ; C12P19/56 ; C12N9/88 ; C12N15/70

Abstract:
The present invention relates a variant polypeptide having kaurenoic acid 13-hydroxylase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 72, 85, 108, 127, 129, 141, 172, 195, 196, 197, 199, 226, 236, 291, 302, 361 or 464, said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
Public/Granted literature
- US20190078105A1 KAURENOIC ACID HYDROXYLASES Public/Granted day:2019-03-14
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