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公开(公告)号：US11781121B2
公开(公告)日：2023-10-10
申请号：US17541565
申请日：2021-12-03
Applicant: DSM IP ASSETS B.V.
CPC classification number: C12N9/1085 , C12N9/0073 , C12N9/88 , C12N15/52 , C12P7/42 , C12P15/00 , C12P19/56 , C12Y114/13078 , C12Y205/01001 , C12Y402/03019
Abstract: The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
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公开(公告)号：US11293043B2
公开(公告)日：2022-04-05
申请号：US16845736
申请日：2020-04-10
Applicant: DSM IP ASSETS B.V.
Inventor： Viktor Marius Boer , Priscilla Zwartjens , Eric Van Den Berg
Abstract: A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 35 or SEQ ID NO: 38 or an amino acid sequence having at least about 50% sequence identity to either thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 35 or SEQ ID NO: 38 or an amino acid sequence having at least about 50% sequence identity to either thereto.
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公开(公告)号：US11913034B2
公开(公告)日：2024-02-27
申请号：US17381479
申请日：2021-07-21
Applicant: DSM IP ASSETS B.V.
CPC classification number: C12N9/0073 , A23L27/36 , C12P19/12 , C12Y114/13079 , A23V2002/00
Abstract: The present invention relates a polypeptide having kaurenoic acid 13-hydroxylase activity, which polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1 or SEQ ID NO: 3, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 136, 248, 336 or 403, said positions being defined with reference to SEQ ID NO: 1 or SEQ ID NO: 3 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
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公开(公告)号：US10760085B2
公开(公告)日：2020-09-01
申请号：US15765549
申请日：2016-10-05
Applicant: DSM IP ASSETS B.V.
Abstract: The present invention relates a variant polypeptide having kaurenoic acid 13-hydroxylase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 72, 85, 108, 127, 129, 141, 172, 195, 196, 197, 199, 226, 236, 291, 302, 361 or 464, said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
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公开(公告)号：US11459548B2
公开(公告)日：2022-10-04
申请号：US17159962
申请日：2021-01-27
Applicant: DSM IP ASSETS B.V.
Inventor： Viktor Marius Boer , Johannes Gustaaf Ernst Van Leeuwen , Priscilla Zwartjens , Catharina Petronella Antonia Maria Kolen
IPC: C12N1/14 , C12N1/16 , C12N1/20 , C12N15/52 , C12N15/63 , C12P5/00 , C12P19/56 , C12N9/10 , A23L27/30 , A23L2/60
Abstract: The present invention relates to a recombinant host comprising a recombinant nucleic acid sequence encoding a polypeptide having at least about: a. 85% identity to the amino acid sequence set forth in SEQ ID NO: 1; b. 85% identity to the amino acid sequence set forth in SEQ ID NO: 3; c. 85% identity to the amino acid sequence set forth in SEQ ID NO: 6; d. 85% identity to the amino acid sequence set forth in SEQ ID NO: 9; e. 85% identity to the amino acid sequence set forth in SEQ ID NO: 11; f. 85% identity to the amino acid sequence set forth in SEQ ID NO: 14; g. 85% identity to the amino acid sequence set forth in SEQ ID NO: 17; h. 85% identity to the amino acid sequence set forth in SEQ ID NO: 20; i. 85% identity to the amino acid sequence set forth in SEQ ID NO: 22; or j. 85% identity to the amino acid sequence set forth in SEQ ID NO: 25.
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Patent Agency Ranking
公开(公告)号：US11178894B2
公开(公告)日：2021-11-23
申请号：US15751674
申请日：2016-08-15
Applicant: DSM IP ASSETS B.V.
Inventor： Viktor Marius Boer , Priscilla Zwartjens , Eric Van Den Berg
IPC: C07K14/39 , C12P19/56 , A23L27/30 , A23L2/60 , C12P15/00 , C07K14/395 , C07K14/40 , C12N1/16 , C12N9/24
Abstract: A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto.
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公开(公告)号：US10947515B2
公开(公告)日：2021-03-16
申请号：US16777427
申请日：2020-01-30
Applicant: DSM IP ASSETS B.V.
Inventor： Viktor Marius Boer , Johannes Gustaaf Emst Van Leeuwen , Priscilla Zwartjens , Catharina Petronella Antonia Maria Kolen
Abstract: The present invention relates to a recombinant host comprising a recombinant nucleic acid sequence encoding a polypeptide having at least about: a. 85% identity to the amino acid sequence set forth in SEQ ID NO: 1; b. 85% identity to the amino acid sequence set forth in SEQ ID NO: 3; c. 85% identity to the amino acid sequence set forth in SEQ ID NO: 6; d. 85% identity to the amino acid sequence set forth in SEQ ID NO: 9; e. 85% identity to the amino acid sequence set forth in SEQ ID NO: 11; f. 85% identity to the amino acid sequence set forth in SEQ ID NO: 14; g. 85% identity to the amino acid sequence set forth in SEQ ID NO: 17; h. 85% identity to the amino acid sequence set forth in SEQ ID NO: 20; i. 85% identity to the amino acid sequence set forth in SEQ ID NO: 22; or j. 85% identity to the amino acid sequence set forth in SEQ ID NO: 25.
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公开(公告)号：US11608364B2
公开(公告)日：2023-03-21
申请号：US17051299
申请日：2019-04-29
Applicant: DSM IP ASSETS B.V.
Inventor： Johannes Gustaaf Ernst Van Leeuwen , Viktor Marius Boer , Priscilla Zwartjens , Jos Van Vugt , René Marcel De Jong
Abstract: The disclosure provides a recombinant cell capable of producing a steviol glycoside, wherein the cell comprises a nucleic acid coding for a variant of a parent polypeptide, wherein the variant has steviol glycoside transport mediating activity, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of the parent polypeptide, comprises at least one modification of the amino acid residue corresponding to any of the amino acids in the amino acid sequence of the parent polypeptide, wherein the variant has an improved ability to produce rebaudioside M and optionally other steviol glycosides extracellularly if compared with the parent polypeptide when measured under the same conditions.
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公开(公告)号：US11225647B2
公开(公告)日：2022-01-18
申请号：US16345129
申请日：2017-10-26
Applicant: DSM IP ASSETS B.V.
Abstract: The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions
92, 100 or 235
said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.-
公开(公告)号：US11203764B2
公开(公告)日：2021-12-21
申请号：US16938483
申请日：2020-07-24
Applicant: DSM IP ASSETS B.V.
Abstract: The present invention relates a variant polypeptide having kaurenoic acid 13-hydroxylase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 72, 85, 108, 127, 129, 141, 172, 195, 196, 197, 199, 226, 236, 291, 302, 361 or 464, said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
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