- 专利标题: Modified guide RNAs, CRISPR-ribonucleotprotein complexes and methods of use
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申请号: US16008376申请日: 2018-06-14
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公开(公告)号: US10907150B2公开(公告)日: 2021-02-02
- 发明人: Jared Matthew Carlson-Stevermer , Krishanu Saha , Amr Ashraf Abdeen , Lucille Katherine Kohlenberg
- 申请人: WISCONSIN ALUMNI RESEARCH FOUNDATION
- 申请人地址: US WI Madison
- 专利权人: WISCONSIN ALUMNI RESEARCH FOUNDATION
- 当前专利权人: WISCONSIN ALUMNI RESEARCH FOUNDATION
- 当前专利权人地址: US WI Madison
- 代理机构: Cantor Colburn LLP
- 主分类号: C12N15/11
- IPC分类号: C12N15/11 ; C12N15/10 ; C12N9/22 ; C12N15/90
摘要:
Described herein are modified guide RNAs such as a single guide RNA including, from 5′ to 3′, a single-stranded protospacer sequence, a first complementary strand of a binding region for the Cas9 polypeptide, an aptamer that binds a biotin-binding molecule, and a second complementary strand of the binding region for the Cas9 polypeptide. Also described is an RNP complex including the modified guide RNA and a Cas9 polypeptide or active fragment thereof. Also included are methods of modifying target genes in cells using the modified guide RNAs.