Invention Grant
- Patent Title: Short-chain nucleic acid elongation primer set, assay kit, and short-chain nucleic acid elongation, amplification and detection methods
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Application No.: US16017367Application Date: 2018-06-25
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Publication No.: US10975433B2Publication Date: 2021-04-13
- Inventor: Keiko Ito , Mika Inada , Koji Hashimoto
- Applicant: KABUSHIKI KAISHA TOSHIBA
- Applicant Address: JP Minato-ku
- Assignee: KABUSHIKI KAISHA TOSHIBA
- Current Assignee: KABUSHIKI KAISHA TOSHIBA
- Current Assignee Address: JP Minato-ku
- Agency: Oblon, McClelland, Maier & Neustadt, L.L.P.
- Priority: JPJP2017-135726 20170711,JPJP2018-083156 20180424
- Main IPC: C12Q1/6876
- IPC: C12Q1/6876 ; C12Q1/6848 ; C12P19/34 ; C12N15/10

Abstract:
According to one embodiment, a primer set for elongating a target short-chain nucleic acid containing a first sequence to obtain an elongated product is provided. The elongated product contains a second, a third, a fourth sequence, a complementary sequence of the 1′-th sequence and a sixth sequence. The complementary sequence of the 1′-th sequence is a loop primer sequence. The primer set contains a first elongation primer containing a first elongation primer sequence and a complementary sequence of the sixth sequence, and a second elongation primer containing a second elongation primer sequence, the fourth, the third, and the second sequence.
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