公开(公告)号：US11952620B2

公开(公告)日：2024-04-09

申请号：US17095139

申请日：2020-11-11

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Koji Hashimoto ,  Keiko Ito ,  Mika Inada

IPC: C12Q1/6851 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/6851 ,  B01L3/5027 ,  B01L3/502715 ,  B01L7/00 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0883 ,  B01L2300/18 ,  C12Q1/6851 ,  C12Q2527/101 ,  C12Q2531/101 ,  C12Q2563/159 ,  C12Q2565/607

Abstract: According to one embodiment, a method of quantifying a target nucleic acid containing a first sequence in a sample is provided. The method includes preparing a substrate on which a plurality of detection regions are arranged, forming a reaction field by placing, on the substrate, a reaction liquid containing a sample, a primer set, and an amplification enzyme, maintaining the reaction field in an isothermal amplification condition, detecting a detection signal for each of the detection regions, determining, for each of the plurality of detection regions, whether positive or negative and detecting or quantifying the target nucleic acid based on the number of positive and/or a rise time of each of the positive detection signal.

公开(公告)号：US20180127814A1

公开(公告)日：2018-05-10

申请号：US15691337

申请日：2017-08-30

Applicant: Kabushiki Kaisha Toshiba

Inventor： Koji HASHIMOTO ,  Keiko Ito ,  Mika Inada

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12N15/09 ,  C12Q1/6823 ,  C12Q1/6825 ,  C12Q1/6851 ,  C12Q2525/301 ,  C12Q2527/101 ,  C12Q2537/1373 ,  C12Q2537/163 ,  C12Q2563/113 ,  C12Q2531/101

Abstract: According to one embodiment, a method for detecting target nucleic acid includes the following steps. (A) A reaction field is formed by placing a reaction mixture on an electrode, and the reaction mixture contains the sample, a primer set, an amplification enzyme, 4 mM to 30 mM of magnesium ion, and a redox probe. The redox probe has an oxidation reduction potential, which generates an electric signal of which amplitude increases. (B) The reaction field is maintained under an amplification reaction condition. (C) The electric signal is detected with the electrode. (D) Existence or quantity of the target nucleic acid is determined.

公开(公告)号：US11353418B2

公开(公告)日：2022-06-07

申请号：US16115870

申请日：2018-08-29

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Koji Hashimoto ,  Keiko Ito ,  Mika Inada

IPC: G01N27/327 ,  B01L3/00 ,  C12Q1/6844 ,  B01L7/00

Abstract: According to one embodiment, a nucleic acid reaction tool includes a support having a first surface, a covering body having a second surface, and a groove opened on the second surface, and a primer set. The covering body is in contact with the support to form a reaction space surrounded by the first surface and the groove. The groove includes, on an inner surface of the reaction space, rising surfaces opposed to each other, and a rear surface connecting one end of the side surfaces, and a primer fixing region to which the primer set is fixed, the primer fixing region being located at a corner where the one end of the side surfaces connected to the rear surface in the reaction space.

公开(公告)号：US20190127793A1

公开(公告)日：2019-05-02

申请号：US16017367

申请日：2018-06-25

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Keiko ITO ,  Mika Inada ,  Koji Hashimoto

IPC: C12Q1/6876 ,  C12N15/10 ,  C12P19/34

Abstract: According to one embodiment, a primer set for elongating a target short-chain nucleic acid containing a first sequence to obtain an elongated product is provided. The elongated product contains a second, a third, a fourth sequence, a complementary sequence of the 1′-th sequence and a sixth sequence. The complementary sequence of the 1′-th sequence is a loop primer sequence. The primer set contains a first elongation primer containing a first elongation primer sequence and a complementary sequence of the sixth sequence, and a second elongation primer containing a second elongation primer sequence, the fourth, the third, and the second sequence.

公开(公告)号：US10975433B2

公开(公告)日：2021-04-13

申请号：US16017367

申请日：2018-06-25

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Keiko Ito ,  Mika Inada ,  Koji Hashimoto

IPC: C12Q1/6876 ,  C12Q1/6848 ,  C12P19/34 ,  C12N15/10

Abstract: According to one embodiment, a primer set for elongating a target short-chain nucleic acid containing a first sequence to obtain an elongated product is provided. The elongated product contains a second, a third, a fourth sequence, a complementary sequence of the 1′-th sequence and a sixth sequence. The complementary sequence of the 1′-th sequence is a loop primer sequence. The primer set contains a first elongation primer containing a first elongation primer sequence and a complementary sequence of the sixth sequence, and a second elongation primer containing a second elongation primer sequence, the fourth, the third, and the second sequence.

公开(公告)号：US10557165B2

公开(公告)日：2020-02-11

申请号：US15691337

申请日：2017-08-30

Applicant: Kabushiki Kaisha Toshiba

Inventor： Koji Hashimoto ,  Keiko Ito ,  Mika Inada

IPC: C12Q1/68 ,  C12Q1/6837 ,  C12Q1/6823 ,  C12Q1/6825 ,  C12Q1/6851 ,  C12N15/09

Abstract: According to one embodiment, a method for detecting target nucleic acid includes the following steps. (A) A reaction field is formed by placing a reaction mixture on an electrode, and the reaction mixture contains the sample, a primer set, an amplification enzyme, 4 mM to 30 mM of magnesium ion, and a redox probe. The redox probe has an oxidation reduction potential, which generates an electric signal of which amplitude increases. (B) The reaction field is maintained under an amplification reaction condition. (C) The electric signal is detected with the electrode. (D) Existence or quantity of the target nucleic acid is determined.

公开(公告)号：US20190285567A1

公开(公告)日：2019-09-19

申请号：US16115870

申请日：2018-08-29

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Koji Hashimoto ,  Keiko Ito ,  Mika Inada

IPC: G01N27/327 ,  B01L3/00 ,  C12Q1/6844

Abstract: According to one embodiment, a nucleic acid reaction tool includes a support having a first surface, a covering body having a second surface, and a groove opened on the second surface, and a primer set. The covering body is in contact with the support to form a reaction space surrounded by the first surface and the groove. The groove includes, on an inner surface of the reaction space, rising surfaces opposed to each other, and a rear surface connecting one end of the side surfaces, and a primer fixing region to which the primer set is fixed, the primer fixing region being located at a corner where the one end of the side surfaces connected to the rear surface in the reaction space.

公开(公告)号：US11655505B2

公开(公告)日：2023-05-23

申请号：US17172378

申请日：2021-02-10

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Keiko Ito ,  Mika Inada ,  Koji Hashimoto

IPC: C12Q1/6876 ,  C12P19/34 ,  C12N15/10 ,  C12Q1/6848

CPC classification number: C12Q1/6876 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/6848 ,  C12Q2600/178 ,  C12Q1/6848 ,  C12Q2525/155 ,  C12Q2525/207 ,  C12Q2525/301 ,  C12Q2531/119

Abstract: According to one embodiment, a primer set for elongating a target short-chain nucleic acid containing a first sequence to obtain an elongated product is provided. The elongated product contains a second, a third, a fourth sequence, a complementary sequence of the 1′-th sequence and a sixth sequence. The complementary sequence of the 1′-th sequence is a loop primer sequence. The primer set contains a first elongation primer containing a first elongation primer sequence and a complementary sequence of the sixth sequence, and a second elongation primer containing a second elongation primer sequence, the fourth, the third, and the second sequence.

公开(公告)号：US11072822B2

公开(公告)日：2021-07-27

申请号：US15908044

申请日：2018-02-28

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Mika Inada ,  Koji Hashimoto ,  Keiko Ito

IPC: C12Q1/68 ,  C12Q1/6853

Abstract: In general, according to one embodiment, a method containing, reverse-transcribing the first sequence in the target RNA, to obtain a reverse-transcription product containing a first′ sequence complementary to the first sequence, dissociating the reverse-transcription product from the target RNA, hybridizing an elongation primer and the reverse-transcription product to elongate both, thereby obtain an elongation product, and maintaining the amplification reaction liquid containing the elongation product, a primer set and Tin(exo-) DNA polymerase and/or Bsm DNA polymerase under an amplification reaction condition, to amplify the first′ sequence.

公开(公告)号：US20180363043A1

公开(公告)日：2018-12-20

申请号：US15907700

申请日：2018-02-28

Applicant: Kabushiki Kaisha Toshiba

Inventor： Koji HASHIMOTO ,  Keiko Ito ,  Mika Inada

IPC: C12Q1/6851 ,  B01L3/00

Abstract: According to one embodiment, a method of quantifying a target nucleic acid containing a first sequence in a sample is provided. The method includes preparing a substrate on which a plurality of detection regions are arranged, forming a reaction field by placing, on the substrate, a reaction liquid containing a sample, a primer set, and an amplification enzyme, maintaining the reaction field in an isothermal amplification condition, detecting a detection signal for each of the detection regions, determining, for each of the plurality of detection regions, whether positive or negative and detecting or quantifying the target nucleic acid based on the number of positive and/or a rise time of each of the positive detection signal.