- Patent Title: Methods for amplifying nucleic acid using tag-mediated displacement
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Application No.: US16195702Application Date: 2018-11-19
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Publication No.: US10982248B2Publication Date: 2021-04-20
- Inventor: Norman C. Nelson , Margarita Batranina-Kaminsky
- Applicant: GEN-PROBE INCORPORATED
- Applicant Address: US CA San Diego
- Assignee: GEN-PROBE INCORPORATED
- Current Assignee: GEN-PROBE INCORPORATED
- Current Assignee Address: US CA San Diego
- Agency: Alston & Bird LLP
- Agent Jeff Landes
- Main IPC: C12Q1/6855
- IPC: C12Q1/6855 ; C12Q1/6844 ; C12P19/34

Abstract:
Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5′ to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5′ to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.
Public/Granted literature
- US20190144906A1 METHODS FOR AMPLIFYING NUCLEIC ACID USING TAG-MEDIATED DISPLACEMENT Public/Granted day:2019-05-16
Information query
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