- 专利标题: Method for immobilizing lectin
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申请号: US16604565申请日: 2018-04-11
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公开(公告)号: US11078254B2公开(公告)日: 2021-08-03
- 发明人: Hiroaki Tateno , Junko Katayama , Kazutaka Matoba , Yoichi Kumada
- 申请人: National Institute of Advanced Industrial Science and Technology , Nissan Chemical Corporation , National University Corporation Kyoto Institute of Technology
- 申请人地址: JP Tokyo; JP Tokyo; JP Kyoto
- 专利权人: National Institute of Advanced Industrial Science and Technology,Nissan Chemical Corporation,National University Corporation Kyoto Institute of Technology
- 当前专利权人: National Institute of Advanced Industrial Science and Technology,Nissan Chemical Corporation,National University Corporation Kyoto Institute of Technology
- 当前专利权人地址: JP Tokyo; JP Tokyo; JP Kyoto
- 代理机构: Mintz Levin Cohn Ferris Glovsky and Popeo, P.C.
- 代理商 Peter F. Corless; Joohee Lee
- 优先权: JPJP2017-078414 20170411
- 国际申请: PCT/JP2018/015163 WO 20180411
- 国际公布: WO2018/190357 WO 20181018
- 主分类号: C07K7/06
- IPC分类号: C07K7/06 ; C07K14/195 ; C07K17/08 ; C07K17/14 ; C07K19/00 ; G01N33/545 ; G01N33/551 ; G01N33/566 ; C07K14/705 ; C07K17/00 ; C12N15/62 ; G01N33/543
摘要:
Provided is a highly sensitive and less expensive lectin-immobilized base material (for example, a lectin plate), such as lectin-immobilized base material having stable qualities and being able to be sufficiently washed after a target sugar chain-containing antigen binds thereto. Further provided is a method for immobilizing lectin to a base material therefor. Particularly provided are: a method whereby a lectin-peptide fusion, in which a peptide capable of adsorbing to a base material surface such as a polystyrene (PS) tag is fused with the N-terminal side or C-terminal side of lectin capable of recognizing a target sugar chain, is immobilized on the peptide side to a base material; and a lectin-immobilized base material produced by this method. By using the lectin-immobilized base material, a target sugar chain-containing antigen can be highly sensitively and evenly measured and, moreover, target sugar chain-containing cells, etc. can be separated (concentrated and harvested).
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