- 专利标题： Enhanced biomolecule detection assays based on tyramide signal amplification and gammaPNA probes
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申请号： US15518811申请日： 2015-10-16
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公开(公告)号： US11124822B2公开(公告)日： 2021-09-21
- 发明人: Bruce Alan Armitage , Veronica Hinman , Munira F. Fouz
- 申请人： CARNEGIE MELLON UNIVERSITY
- 申请人地址： US PA Pittsburgh
- 专利权人： CARNEGIE MELLON UNIVERSITY
- 当前专利权人： CARNEGIE MELLON UNIVERSITY
- 当前专利权人地址： US PA Pittsburgh
- 代理机构： The Webb Law Firm
- 国际申请： PCT/US2015/055949 WO 20151016
- 国际公布： WO2016/061460 WO 20160421
- 主分类号： C12Q1/6841
- IPC分类号： C12Q1/6841 ; C12Q1/682 ; C12Q1/6816 ; C12Q1/6818
摘要：
Provided herein are methods of detecting target analytes, such as nucleic acids, for example microRNAs using an enhanced Tyramide Signal Amplification (TSA) method that employs probes tagged with tyramide-binding groups to amplify the effects of the TSA. The accessibility of the tyramide-binding groups, such as hydroxyphenyl groups, provides for large improvements in signal due to faster reaction with the radicals. The present invention further includes the application of the assay for detecting specific microRNAs.
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