公开(公告)号：US11124822B2

公开(公告)日：2021-09-21

申请号：US15518811

申请日：2015-10-16

申请人： CARNEGIE MELLON UNIVERSITY

发明人： Bruce Alan Armitage ,  Veronica Hinman ,  Munira F. Fouz

IPC分类号： C12Q1/6841 ,  C12Q1/682 ,  C12Q1/6816 ,  C12Q1/6818

摘要： Provided herein are methods of detecting target analytes, such as nucleic acids, for example microRNAs using an enhanced Tyramide Signal Amplification (TSA) method that employs probes tagged with tyramide-binding groups to amplify the effects of the TSA. The accessibility of the tyramide-binding groups, such as hydroxyphenyl groups, provides for large improvements in signal due to faster reaction with the radicals. The present invention further includes the application of the assay for detecting specific microRNAs.

公开(公告)号：US20180142288A1

公开(公告)日：2018-05-24

申请号：US15802066

申请日：2017-11-02

申请人： CARNEGIE MELLON UNIVERSITY

发明人： Munira F. Fouz ,  Krzysztof Matyjaszewski ,  Bruce A. Armitage ,  Subha Ranjan Das ,  Saadyah Averick

IPC分类号： C12Q1/6834 ,  C07H21/04 ,  C08L57/00

CPC分类号： C12Q1/6834 ,  A61K47/6883 ,  C07H21/04 ,  C08F2438/01 ,  C08L57/00 ,  C08L57/12 ,  C08L2203/02 ,  C08L2205/14 ,  G01N33/582

摘要： A composition includes a polymer including extending chains, side chains, or branches. One (or more) of a plurality of a first strand of nucleic acid is attached to each of a plurality of the side chains. One (or more) of a plurality of a second strand of nucleic acid, which is complementary to the first strand of nucleic acid, is complexed to each of the plurality of the first strand of nucleic acid to form a double strand of nucleic acid on each of the plurality of the side chains. At least one fluorescent compound is associated with the double strand of nucleic acid on each of the plurality of the side chains.

公开(公告)号：US10982266B2

公开(公告)日：2021-04-20

申请号：US15802066

申请日：2017-11-02

申请人： CARNEGIE MELLON UNIVERSITY

发明人： Munira F. Fouz ,  Krzysztof Matyjaszewski ,  Bruce A. Armitage ,  Subha Ranjan Das ,  Saadyah Averick

IPC分类号： C12Q1/68 ,  C12Q1/6834 ,  C07H21/04 ,  C08L57/00 ,  G01N33/58 ,  C08L57/12 ,  A61K47/68

摘要： A composition includes a polymer including extending chains, side chains, or branches. One (or more) of a plurality of a first strand of nucleic acid is attached to each of a plurality of the side chains. One (or more) of a plurality of a second strand of nucleic acid, which is complementary to the first strand of nucleic acid, is complexed to each of the plurality of the first strand of nucleic acid to form a double strand of nucleic acid on each of the plurality of the side chains. At least one fluorescent compound is associated with the double strand of nucleic acid on each of the plurality of the side chains.