专利检索 ap:("CARNEGIE MELLON UNIVERSITY") AND inv:"Bruce Alan Armitage" 第 1 页

1.

发明授权
Enhanced biomolecule detection assays based on tyramide signal amplification and gammaPNA probes 有权

公开(公告)号：US11124822B2

公开(公告)日：2021-09-21

申请号：US15518811

申请日：2015-10-16

申请人： CARNEGIE MELLON UNIVERSITY

发明人： Bruce Alan Armitage , Veronica Hinman , Munira F. Fouz

IPC分类号： C12Q1/6841 , C12Q1/682 , C12Q1/6816 , C12Q1/6818

摘要： Provided herein are methods of detecting target analytes, such as nucleic acids, for example microRNAs using an enhanced Tyramide Signal Amplification (TSA) method that employs probes tagged with tyramide-binding groups to amplify the effects of the TSA. The accessibility of the tyramide-binding groups, such as hydroxyphenyl groups, provides for large improvements in signal due to faster reaction with the radicals. The present invention further includes the application of the assay for detecting specific microRNAs.