A USB interface detection module includes a detection unit and an adapting device. The adapting device is electrically connected between a first electronic device and a second electronic device. According to the interface specification of the first electronic device, the voltage value of a configuration channel pin is changeable through the use of the GND_DRAIN pin and the switching action of a field-effect transistor switch. Consequently, the interface specification of the first electronic device is acquired by the second electronic device.