Methods and apparatus for video coding are disclosed. According to one method, a bitstream is generated or received, where the bitstream includes a first syntax and a second syntax. The first syntax is related to a target number of bits used to represent a set of third syntaxes and each third syntax specifies one subpicture ID for one subpicture in a set of subpictures. The second syntax is related to a total number of subpictures in the set of subpicture, where a first number that can be represented by the target number of bits is equal to or greater than the total number of subpictures. According to another method, the subpicture ID syntaxes have different values for different subpictures.