- 专利标题: Apparatus for efficient genetic modification of cells
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申请号: US16137478申请日: 2018-09-20
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公开(公告)号: US11584908B2公开(公告)日: 2023-02-21
- 发明人: Kenneth T. Kotz , Bryan D. Teece , James Gillett Truslow , Nathan Francis Moore , Jeffrey T. Borenstein , Vishal Tandon
- 申请人: THE CHARLES STARK DRAPER LABORATORY, INC.
- 申请人地址: US MA Cambridge
- 专利权人: THE CHARLES STARK DRAPER LABORATORY, INC.
- 当前专利权人: THE CHARLES STARK DRAPER LABORATORY, INC.
- 当前专利权人地址: US MA Cambridge
- 代理机构: Foley & Lardner LLP
- 主分类号: C12M1/02
- IPC分类号: C12M1/02 ; C12M1/12 ; C12N15/87 ; C12M1/00 ; C12N15/86 ; C12M3/06 ; C12M1/40 ; C12M1/34 ; A61K48/00
摘要:
A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.
公开/授权文献
- US20190085280A1 APPARATUS FOR EFFICIENT GENETIC MODIFICATION OF CELLS 公开/授权日:2019-03-21