An autosampler includes an injection port for supplying a sample to an analysis device, a needle that collects the sample stored in a vial and injects the sample into the injection port, a cleaning unit that cleans the needle and a gas exhaust fan. The cleaning unit includes a cleaning container which stores a cleaning liquid and into which the needle is inserted, and a unit main body that has a space for storing the cleaning container and receiving the cleaning liquid overflowing from the cleaning container, and a unit gas exhaust passage for exhausting gas in the space outwardly of the cleaning unit. The unit main body has a unit opening through which the needle passes when accessing the cleaning container. The autosampler includes a boundary portion that separates a first region in which the injection port, the needle and the unit opening are arranged from a second region in which the gas exhaust fan is arranged, and a gas exhaust region which is connected to the unit gas exhaust passage via a gas exhaust opening provided at the boundary portion and into which gas exhausted from the space flows due to a negative pressure generated by the gas exhaust fan.