Invention Grant
- Patent Title: Embedding medium of biological sample for its imaging by light and/or electron microscopy
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Application No.: US16980560Application Date: 2019-03-15
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Publication No.: US11860074B2Publication Date: 2024-01-02
- Inventor: Xavier Heiligenstein
- Applicant: CRYOCAPCELL
- Applicant Address: FR Paris
- Assignee: CRYOCAPCELL
- Current Assignee: CRYOCAPCELL
- Current Assignee Address: FR Paris
- Agency: Maier & Maier, PLLC
- Priority: EP 305293 2018.03.16
- International Application: PCT/EP2019/056584 2019.03.15
- International Announcement: WO2019/175407A 2019.09.19
- Date entered country: 2020-09-14
- Main IPC: G01N1/36
- IPC: G01N1/36 ; C08F265/06 ; C08K5/098 ; G02B21/33 ; H01J37/20

Abstract:
The present invention relates to the field of microscopy, preferably electron microscopy. Especially, the present invention concerns an embedding medium for imaging a biological sample by microscopy comprising:
from 60% to 99% wt. of a glycol dimethacrylate selected from alkylene glycol dimethacrylate and/or oligo(alkylene glycol) dimethacrylate;
from 0% to 38% wt. of a polyalkylene glycol diacrylate or of a polyalkylene glycol methacrylate; said polyalkylene glycol diacrylate or polyalkylene glycol methacrylate being optionally substituted by at least one hydrophilic group such as hydroxyl, amino, or an oxo group;
at least one additive, preferably comprising at least one heavy metal salt or lanthanide salt; and
from 0.1% to 2% wt. of a radical polymerization initiator.
The present invention also refers to the electro-conductive material resulting from the polymerization of the embedding medium of the invention, and the process and kits of preparation thereof; said material embedding at least one biological sample.
The present invention also relates to a method for imaging by microscopy, a biological sample comprising using the embedding medium and/or the electro-conductive material of the invention.
from 60% to 99% wt. of a glycol dimethacrylate selected from alkylene glycol dimethacrylate and/or oligo(alkylene glycol) dimethacrylate;
from 0% to 38% wt. of a polyalkylene glycol diacrylate or of a polyalkylene glycol methacrylate; said polyalkylene glycol diacrylate or polyalkylene glycol methacrylate being optionally substituted by at least one hydrophilic group such as hydroxyl, amino, or an oxo group;
at least one additive, preferably comprising at least one heavy metal salt or lanthanide salt; and
from 0.1% to 2% wt. of a radical polymerization initiator.
The present invention also refers to the electro-conductive material resulting from the polymerization of the embedding medium of the invention, and the process and kits of preparation thereof; said material embedding at least one biological sample.
The present invention also relates to a method for imaging by microscopy, a biological sample comprising using the embedding medium and/or the electro-conductive material of the invention.
Public/Granted literature
- US20210003487A1 EMBEDDING MEDIUM OF BIOLOGICAL SAMPLE FOR ITS IMAGING BY LIGHT AND/OR ELECTRON MICROSCOPY Public/Granted day:2021-01-07
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