公开(公告)号：US11860074B2

公开(公告)日：2024-01-02

申请号：US16980560

申请日：2019-03-15

Applicant: CRYOCAPCELL

Inventor： Xavier Heiligenstein

IPC: G01N1/36 ,  C08F265/06 ,  C08K5/098 ,  G02B21/33 ,  H01J37/20

CPC classification number: G01N1/36 ,  C08F265/06 ,  C08K5/098 ,  G02B21/33 ,  H01J37/20 ,  G01N2001/364

Abstract: The present invention relates to the field of microscopy, preferably electron microscopy. Especially, the present invention concerns an embedding medium for imaging a biological sample by microscopy comprising:



from 60% to 99% wt. of a glycol dimethacrylate selected from alkylene glycol dimethacrylate and/or oligo(alkylene glycol) dimethacrylate;
from 0% to 38% wt. of a polyalkylene glycol diacrylate or of a polyalkylene glycol methacrylate; said polyalkylene glycol diacrylate or polyalkylene glycol methacrylate being optionally substituted by at least one hydrophilic group such as hydroxyl, amino, or an oxo group;
at least one additive, preferably comprising at least one heavy metal salt or lanthanide salt; and
from 0.1% to 2% wt. of a radical polymerization initiator.




The present invention also refers to the electro-conductive material resulting from the polymerization of the embedding medium of the invention, and the process and kits of preparation thereof; said material embedding at least one biological sample.
The present invention also relates to a method for imaging by microscopy, a biological sample comprising using the embedding medium and/or the electro-conductive material of the invention.

公开(公告)号：US11650007B2

公开(公告)日：2023-05-16

申请号：US17264942

申请日：2019-08-14

Applicant: CRYOCAPCELL

Inventor： Jérôme Heiligenstein ,  Xavier Heiligenstein

IPC: F25D29/00 ,  F25D3/10

CPC classification number: F25D29/001 ,  F25D3/105 ,  F25D2400/30

Abstract: This cryogenic preservation or vitrification device (1) comprises:



an accumulator (2) including a first volume of pressurised hydraulic oil;
a control system controlling a valve (20) capable of releasing a second volume of hydraulic oil from the accumulator toward a cylinder (55) by means of a pipe (4);
a cylinder (55) including a piston (6) configured to be driven by the second volume of hydraulic oil and to displace a first volume of cryogenic fluid to a cryogenic vessel (7) intended to receive a sample (8) to be cryogenically preserved.

公开(公告)号：US20210302094A1

公开(公告)日：2021-09-30

申请号：US17264942

申请日：2019-08-14

Applicant: CRYOCAPCELL

Inventor： Jérôme HEILIGENSTEIN ,  Xavier HEILIGENSTEIN

IPC: F25D29/00 ,  F25D3/10

Abstract: A cryogenic preservation or vitrification device that has an accumulator including a first volume of pressurised hydraulic oil; a control system controlling a valve capable of releasing a second volume of hydraulic oil from the accumulator toward a cylinder by use of a pipe; and a cylinder including a piston configured to be driven by the second volume of hydraulic oil and to displace a first volume of cryogenic fluid to a cryogenic vessel intended to receive a sample to be cryogenically preserved.

公开(公告)号：US20210003487A1

公开(公告)日：2021-01-07

申请号：US16980560

申请日：2019-03-15

Applicant: CRYOCAPCELL

Inventor： Xavier HEILIGENSTEIN

IPC: G01N1/36 ,  C08F265/06 ,  C08K5/098 ,  H01J37/20 ,  G02B21/33

Abstract: The present invention relates to the field of microscopy, preferably electron microscopy. Especially, the present invention concerns an embedding medium for imaging a biological sample by microscopy comprising: from 60% to 99% wt. of a glycol dimethacrylate selected from alkylene glycol dimethacrylate and/or oligo(alkylene glycol) dimethacrylate; from 0% to 38% wt. of a polyalkylene glycol diacrylate or of a polyalkylene glycol methacrylate; said polyalkylene glycol diacrylate or polyalkylene glycol methacrylate being optionally substituted by at least one hydrophilic group such as hydroxyl, amino, or an oxo group; at least one additive, preferably comprising at least one heavy metal salt or lanthanide salt; and from 0.1% to 2% wt. of a radical polymerization initiator. The present invention also refers to the electro-conductive material resulting from the polymerization of the embedding medium of the invention, and the process and kits of preparation thereof; said material embedding at least one biological sample.
The present invention also relates to a method for imaging by microscopy, a biological sample comprising using the embedding medium and/or the electro-conductive material of the invention.