A cover glass transfer device for a sample sealing apparatus performs picking up a cover glass (CG), one at a time, and sealing a cell sample piece placed on a glass slide. A plurality of CGs are stacked in layers at an angle. An elevating member movable up and down with respect to the CG is provided with a holding rod and a push rod which are biased downward by a spring and are movably inserted therethrough. A suction face of a suction pad fixed on the lower end of the holding rod is disposed above the lower end of the push rod in a state where the rods are stretched out downward. The suction pad comes into contact with and holds by suction the top CG upon the descent of the elevating member following the holding rod. Due to the ascent of the elevating member, an upward force caused by the holding rod and a downward force caused by the push rod work on the top CG. Then, a downward convex deflection is provided to the CG to change a curvature thereof in a state where one end of the CG is in contact with the adjacent lower CG, thereby separating the CG off from the adjacent lower CG.