发明申请
- 专利标题: Cell Death Inducing Agents
- 专利标题(中): 细胞死亡诱导剂
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申请号: US10582304申请日: 2004-12-10
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公开(公告)号: US20070280951A1公开(公告)日: 2007-12-06
- 发明人: Naoki Kimura , Masayuki Tsuchiya , Masahiko Nanami , Takashi Tomimatsu , Shigeto Kawai
- 申请人: Naoki Kimura , Masayuki Tsuchiya , Masahiko Nanami , Takashi Tomimatsu , Shigeto Kawai
- 优先权: JP2003-415758 20031212
- 国际申请: PCT/JP04/18501 WO 20041210
- 主分类号: A61K39/395
- IPC分类号: A61K39/395 ; A61P35/00 ; A61P37/00 ; C07H21/00 ; C07K16/00 ; C12N1/00 ; C12N15/63 ; C12N5/00 ; C12P21/00
摘要:
The present inventors constructed a DNA expression vector encoding 2D7sc(Fv)2 in which the heavy chain variable region sequence (VH) and the light chain variable region sequence (VL) of the 2D7 antibody is arranged in the order of VH-VL-VH-VL, and these sequences are linked by a 15-mer linker. The vector was introduced into CHO cells and a 2D7sc(Fv)2-producing expression cell line was established. When 2D7sc(Fv)2 was expressed in this cell line, purified, and cell death-inducing experiments performed, 2D7sc(Fv)2 was found to have a concentration-dependent cell death-inducing activity.
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