发明申请
- 专利标题： Method for improving enzymatic activity of glycosyltransferases
- 专利标题（中）： 改善糖基转移酶酶活性的方法
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申请号： US11665568申请日： 2005-09-30
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公开(公告)号： US20090087894A1公开(公告)日： 2009-04-02
- 发明人: Hiroshi Tsukamoto , Takeshi Yamamoto , Yoshimitsu Takakura
- 申请人： Hiroshi Tsukamoto , Takeshi Yamamoto , Yoshimitsu Takakura
- 申请人地址： JP Tokyo
- 专利权人： Japan Tobacco Inc.
- 当前专利权人： Japan Tobacco Inc.
- 当前专利权人地址： JP Tokyo
- 优先权： JPPCT/JP2004/015363 20041018
- 国际申请： PCT/JP05/18169 WO 20050930
- 主分类号： C12N9/10
- IPC分类号： C12N9/10
摘要：
The present invention provides an inexpensive and simple method which allows efficient glycosylation with glycosyltransferases derived from microorganisms of the Vibrionaceae family when compared to conventional enzymatic reaction systems.According to the method of the present invention, glycosyltransferases derived from microorganisms of the Vibrionaceae, such as β-galactoside-α2,6-sialyltransferase derived from Photobacterium damselae, β-galactoside-α2,3-sialyltransferase derived from Photobacterium phosphoreum and β-galactoside-α2,3-sialyltransferase derived from Vibrio sp., enhance their enzymatic activity when an appropriate amount of NaCl is added to their enzyme reaction systems.
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